bs-10129R [Primary Antibody]
P21 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P21

Immunogen Range: 21-100/164


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1026

Swiss Prot: P38936

Source: KLH conjugated synthetic peptide derived from human P21

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 18


Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
  • Shi, Lei, et al. "Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro." Theriogenology (2017).Read more>>
  • Wang, Ping, et al. "Cisplatin induces HepG2 cell cycle arrest through targeting specific long noncoding RNAs and the p53 signaling pathway." Oncology Letters 12.6 (2016): 4605-4612.Read more>>
  • Cho S et al. Enhanced Anticancer Effect of Adding Magnesium to Vitamin C Therapy: Inhibition of Hormetic Response by SVCT-2 Activation. Transl Oncol. 2019 Dec 31;13(2):401-409. Read more>>
  • Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
  • Xinchen Wang. et al. Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics. Int J Mol Sci. 2021 Jan;22(22):12415Read more>>
  • Cuifang Chang. et al. The orphan GPR50 receptor interacting with T_RI induces G1/S-phase cell cycle arrest via Smad3-p27/p21 in BRL-3A cells. BIOCHEM PHARMACOL. 2022 Aug;202:115117Read more>>
  • Jia Wang. et al. FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice. INT J MOL SCI. 2022 Jan;23(23):14979Read more>>
  • Niuxin Yang. et al. Identification of Senescent Cells in Peri-Implantitis and Prevention of Mini-Implant Loss Using Senolytics. INT J MOL SCI. 2023 Jan;24(3):2507Read more>>
  • Wenqi Deng. et al. A Senomorphic-Conjugated Scaffold for Application of Senescent Cells in Regenerative Medicine. Advanced Therapeutics. 2023 Feb;:2200276Read more>>
  • Wenqi DENG. et al. Senescence-associated secretory phenotypes in rat-derived dedifferentiated fat cells with replicative senescence. DENT MATER J. 2023 Feb 1Read more>>
  • Haitao LIU. et al. Localization of senescent cells under cavity preparations in rats and restoration of reparative dentin formation by senolytics. DENT MATER J. 2023 Mar 01Read more>>
  • Xinchen Wang. et al. Senolytics ameliorate the failure of bone regeneration through the cell senescence-related inflammatory signalling pathway. BIOMED PHARMACOTHER. 2024 Jun;175:116606Read more>>
  • Chuyi LUO. et al. Detection of senescent cells in the mucosal healing process on type 2 diabetic rats after tooth extraction for biomaterial development. DENT MATER J. 2024 Apr 19Read more>>
  • Kramer Naomi E. et al. Insights into Brominated Flame Retardant Neurotoxicity: Mechanisms of Hippocampal Neural Cell Death and Brain Region-Specific Transcriptomic Shifts in Mice. TOXICOL SCI. 2024 JulRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Human MG-63 lysates probed with Rabbit Anti-P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


MCF-7 cells were stained with P21 Polyclonal Antibody, Unconjugated(bs-10129R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Paraformaldehyde-fixed, paraffin-embedded Human Hepatoma tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CDKN1A/P21) Polyclonal Antibody, Unconjugated (bs-10129R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.