bs-10129R-Cy5 [Conjugated Primary Antibody]
P21 Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P21

Immunogen Range: 21-100/164


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1026

Swiss Prot: P38936

Source: KLH conjugated synthetic peptide derived from human P21

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 18


Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
  • Shi, Lei, et al. "Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro." Theriogenology (2017).Read more>>
  • Wang, Ping, et al. "Cisplatin induces HepG2 cell cycle arrest through targeting specific long noncoding RNAs and the p53 signaling pathway." Oncology Letters 12.6 (2016): 4605-4612.Read more>>
  • Cho S et al. Enhanced Anticancer Effect of Adding Magnesium to Vitamin C Therapy: Inhibition of Hormetic Response by SVCT-2 Activation. Transl Oncol. 2019 Dec 31;13(2):401-409. Read more>>
  • Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
  • Xinchen Wang. et al. Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics. Int J Mol Sci. 2021 Jan;22(22):12415Read more>>
  • Cuifang Chang. et al. The orphan GPR50 receptor interacting with T_RI induces G1/S-phase cell cycle arrest via Smad3-p27/p21 in BRL-3A cells. BIOCHEM PHARMACOL. 2022 Aug;202:115117Read more>>
  • Jia Wang. et al. FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice. INT J MOL SCI. 2022 Jan;23(23):14979Read more>>
  • Niuxin Yang. et al. Identification of Senescent Cells in Peri-Implantitis and Prevention of Mini-Implant Loss Using Senolytics. INT J MOL SCI. 2023 Jan;24(3):2507Read more>>
  • Wenqi Deng. et al. A Senomorphic-Conjugated Scaffold for Application of Senescent Cells in Regenerative Medicine. Advanced Therapeutics. 2023 Feb;:2200276Read more>>
  • Wenqi DENG. et al. Senescence-associated secretory phenotypes in rat-derived dedifferentiated fat cells with replicative senescence. DENT MATER J. 2023 Feb 1Read more>>
  • Haitao LIU. et al. Localization of senescent cells under cavity preparations in rats and restoration of reparative dentin formation by senolytics. DENT MATER J. 2023 Mar 01Read more>>
VALIDATION IMAGES