bs-10152R [Primary Antibody]
Desmoglein 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Desmoglein 2

Immunogen Range: 51-150/1118


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1829

Swiss Prot: Q14126

Source: KLH conjugated synthetic peptide derived from human Desmoglein 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 122


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. Read more>>
  • Qing Wang. et al. Desmosomal Junctions and Connexin-43 Remodeling in High-Pacing-Induced Heart Failure Dogs. ANATOL J CARDIOL. 2023 Aug; 27(8): 462C471Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded labeled with Anti-Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.