Host: Rabbit
Target Protein: c-fos
Immunogen Range: 145-260/380
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2353
Swiss Prot: P01100
Source: KLH conjugated synthetic peptide derived from human c-fos
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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- Zhang, Fengwei, et al. "Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus)." Scientific Reports 7.1 (2017): 16627.Read more>>
- Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer\induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068Read more>>
- Zhao, Lifang. et al. Real-world PM2.5 exposure induces pathological injury and DNA damage associated with miRNAs and DNA methylation alteration in rat lungs. Environ Sci Pollut R. 2022 Jan;:1-16Read more>>
- Tao Chen. et al. The pathological involvement of spinal cord EphB2 in visceral sensitization in male rats. STRESS. 2022;25(1):166-178Read more>>
- Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678Read more>>
- Lu Peng. et al. The effect of c-Fos on the prognosis, proliferation, and invasion of oral squamous cell carcinoma. ORAL DIS. 2024 FebRead more>>
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-10172R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-10172R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-10172R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-10172R) at 1:400 overnight at 4°C, DAB staining.