bs-10183R [Primary Antibody]
CMKLR1 Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: CMKLR1

Immunogen Range: 171-280/373


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1240

Source: KLH conjugated synthetic peptide derived from human CMKLR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Chemokine-Like Receptor 1 (CMKLR1) is a serpentine transmembrane G-protein linked receptor. It is an orphan chemoattractant receptor, also known as ChemR23 or DEZ. CMKLR1 is expressed on the surface of monocyte-derived immature dendritic cells and peripheral plamacytoid dendritic (pDC) cells; but not on myeloid dendritic (mDC) cells, monocytes and lymphocytes. The expression of CMKLR1 on pDC is down-regulated after activation. Chemerin (TIG2) is a natural ligand of CMKLR1. The interaction of CMKLR1 with Chemerin induces pDC activation and chemotaxis. It has been shown that CMKLR1 is a coreceptor for several strains of SIV and HIV-1. CMKLR1 is a potential useful marker for distinguishing pDCs and mDCs.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Dog
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Alinor Hanthaziet al. Chemerin Added to Endothelin-1 Promotes Rat Pulmonary Artery Smooth Muscle Cell Proliferation and Migration. Front Physiol. 2020 Jul 30;11:926.Read more>>
  • Ye Y et al. Chemerin/ChemR23 regulates cementoblast function and tooth resorption in mice via inflammatory factorsJ Periodontol.2020 Dec 1.Read more>>
VALIDATION IMAGES

Mouse brain lysates probed with Anti-CMKLR1 Polyclonal Antibody (bs-10183R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human HepG2 cell lysates probed with CMKLR1 Polyclonal Antibody, Unconjugated (bs-10183R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.