bs-10188R [Primary Antibody]
Endothelial Lipase Polyclonal Antibody
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Host: Rabbit

Target Protein: Endothelial Lipase

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9388

Swiss Prot: Q9Y5X9

Source: recombinanted human Endothelial lipase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.

Size: 100µL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pawe? Jan Stanirowski. et al. Expression of Placental Lipid Transporters in Pregnancies Complicated by Gestational and Type 1 Diabetes Mellitus. INT J MOL SCI. 2024 Jan;25(6):3559Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse placenta labeled with Rabbit Anti-Endothelial Lipase Polyclonal Antibody, Unconjugated (bs-10188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Endothelial Lipase Polyclonal Antibody, Unconjugated (bs-10188R) at 1:200 overnight at 4°C, DAB staining.