bs-10196R [Primary Antibody]
Pan-Actin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Actin

Specificity: Alpha, Beta and Gamma Actins

Immunogen Range: 165-260/377


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 70

Swiss Prot: P68032

Source: KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat
Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han L et al. Interleukin-1β-Induced Senescence Promotes Osteoblastic Transition of Vascular Smooth Muscle Cells. Kidney Blood Press Res. 2020;45(2):314-330. Read more>>
  • Li, Liucheng, et al. "Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats." Phytomedicine 22.1 (2015): 111-119.Read more>>
  • Qiao, Xi, et al. "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction."Nephrology (2015).Read more>>
  • Yan, Xuerui, et al. "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-α-Treated Rat Aortic Smooth Muscle Cells." Cardiovascular Toxicology (2015): 1-9.Read more>>
  • Pang, X-X., et al. "Urotensin II Induces ER Stress and EMT and Increase Extracellular Matrix Production in Renal Tubular Epithelial Cell in Early Diabetic Mice." Kidney and Blood Pressure Research 41.4 (2016): 434-449.Read more>>
  • Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.Read more>>
  • Zhou et al. Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration. (2017) Mol.Med.Re. 15:4123-4131Read more>>
  • Kim et al. Vandetanib and ADAM inhibitors synergistically attenuate the pathological migration of EBV-infected retinal pigment epithelial cells by regulating the VEGF-mediated MAPK pathway. (2017) Exp.Ther.Me. 13:1415-1425Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Liu et al. Therapeutic effects of suppressors of cytokine signaling in diabetic nephropathy. (2014) J.Histochem.Cytoche. 62:119-28Read more>>
  • Gan et al. Preventive effects of ¹²⁵I seeds on benign restenosis following esophageal stent implantation in a dog model. (2015) Mol.Med.Re. 11:3382-90Read more>>
  • Park and Kim Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway. (2018) Mol.Med.Rep. 17:3563-3574Read more>>
  • Ouyang et al. Downregulation of microRNA-429 inhibits cell proliferation by targeting p27Kip1 in human prostate cancer cells. (2015) Mol.Med.Rep. 11:1435-41Read more>>
  • Wang et al. MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2. (2017) Cell.Death.Dis. 8:e2792Read more>>
  • Zeng Y et al. Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.(2018) Lipids in Health and Disease, Nov 6;17(1):251. Read more>>
  • Lu XY et al.Codonopsis tangshen Oliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with Streptozotocin. (2018)Nat Prod Bioprospect.8(6):441-451.Read more>>
  • Jiang L et al. Detecting Vulnerable Atherosclerotic Plaques by 68Ga-Labeled Divalent Cystine Knot Peptide. Mol Pharm. 2019 Mar 4;16(3):1350-1357. Read more>>
  • Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2. Read more>>
  • Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.Read more>>
  • Cai YH et al. Riboflavin/ultraviolet A-induced collagen cross-linking in rabbit corneal scar. Int J Ophthalmol. 2019 Jan 18;12(1):46-50. Read more>>
  • Mao Q et al. Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF‑β1/Smad/miR‑192 signaling pathway. EXPERIMENTAL AND THERAPEUTIC MEDICINE.2019 18: 3053-3061.Read more>>
  • Wu W et al. Yu Jin Pulvis inhibits carbon tetrachloride-induced liver fibrosis by blocking the MAPK and PI3K/Akt signaling pathways. Am J Transl Res. 2019 Sep 15;11(9):5998-6006. eCollection 2019.Read more>>
  • Zhang Y et al. Estrogen facilitates gastric cancer cell proliferation and invasion through promoting the secretion of interleukin-6 by cancer-associated fibroblasts. Int Immunopharmacol. 2019 Nov 18:105937. Read more>>
  • Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.Read more>>
  • Zhao H et al. miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1. Toxicol Appl Pharmacol. 2019 Nov 14;386:114827. Read more>>
  • Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927. Read more>>
  • Hou L et al. Nontoxic concentration of ochratoxin A decreases the dosage of cyclosporine A to induce chronic nephropathy model via autophagy mediated by toll-like receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153. Read more>>
  • Han L et al. Interleukin-1β-Induced Senescence Promotes Osteoblastic Transition of Vascular Smooth Muscle Cells. Kidney Blood Press Res. 2020;45(2):314-330. Read more>>
  • Hou L et al. Nontoxic Concentration of Ochratoxin A Decreases the Dosage of Cyclosporine A to Induce Chronic Nephropathy Model via Autophagy Mediated by Toll-Like Receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153.Read more>>
  • Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-B signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516.Read more>>
  • Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
  • Jing Wang et al. Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD. Mediators Inflamm. 2020 May 8;2020:4260204.Read more>>
  • Wan Q et al. Overexpression of laminin 4 facilitates proliferation and migration of fibroblasts in knee arthrofibrosis by targeting canonical Shh/Gli1 signaling. Connect Tissue Res. 2020 Jun 10;1-11.Read more>>
  • Min Jianget al. P2X7R orchestrates the progression of murine hepatic fibrosis by making a feedback loop from macrophage to hepatic stellate cells. Toxicol Lett. 2020 Oct 15;333:22-32.Read more>>
  • Gong Y et al. Smad3 C-terminal phosphorylation site mutation attenuates the hepatoprotective effect of salvianolic acid B against hepatocarcinogenesisFood Chem Toxicol.2021 Jan;147:111912.Read more>>
  • Chen D et al. Sodium Propionate Attenuates the Lipopolysaccharide-Induced EpithelialCMesenchymal Transition via the PI3K/Akt/mTOR Signaling Pathway. J Agric Food Chem. 2020 Jun 17;68(24):6554-6563.Read more>>
  • Han X et al. The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-beta1/Smad3 pathway. Biomed Pharmacother.2020 Dec 24;134:111135.Read more>>
  • Jianwei Fan. et al. Baicalin Ameliorates Pancreatic Fibrosis by Inhibiting the Activation of Pancreatic Stellate Cells in Mice with Chronic Pancreatitis. Front Pharmacol. 2020; 11: 607133Read more>>
  • Chaoqun Hu. et al. Urine-derived stem cells accelerate the recovery of injured mouse hepatic tissue. Am J Transl Res. 2020; 12(9): 5131C515Read more>>
  • Zheng XP. et al. Kidney-targeted baicalin-lysozyme conjugate ameliorates renal fibrosis in rats with diabetic nephropathy induced by streptozotocin.. Bmc Nephrol. 2020 May;21(1):174-174Read more>>
  • Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-B and TGF-/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275Read more>>
  • Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895C6907Read more>>
  • Wen-qian Jia. et al. CD73 regulates hepatic stellate cells activation and proliferation through Wnt/-catenin signaling pathway. Eur J Pharmacol. 2021 Jan;890:173667Read more>>
  • Qionghua Hu. et al. Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis. J Surg Res. 2021 Sep;265:223Read more>>
  • Xueli Li. et al. Dynamic expression of vascular endothelial growth factor (VEGF) and platelet-derived growth factor receptor beta (PDGFR) in diabetic brain contributes to cognitive dysfunction. Brain Res Bull. 2021 Oct;175:99Read more>>
  • Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634Read more>>
  • Xiaohui Chen. et al. Eleutheroside B-loaded poly (lactic-co-glycolic acid) nanoparticles protect against renal fibrosis via Smad3-dependent mechanism. 2021 Sep 28Read more>>
  • Ms. Nannan Zhang. et al. Characterization of Urine Derived Stem Cells from Patients with End-Stage Liver Diseases and Application to Induced Acute and Chronic Liver Injury of Nude Mice Model. 2021 Sep 22Read more>>
  • Hong Zhu. et al. Sennoside A alleviates inflammatory responses by inhibiting the hypermethylation of SOCS1 in CCl4-induced liver fibrosis. Pharmacol Res. 2021 Oct;:105926Read more>>
  • Han Ding. et al. JQ-1 ameliorates schistosomiasis liver fibrosis by suppressing JAK2 and STAT3 activation. Biomed Pharmacother. 2021 Dec;144:112281Read more>>
VALIDATION IMAGES

Human A549 lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


HeLa cells probed with Pan-Actin Polyclonal Antibody, unconjugated (bs-10196R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)


Lane 1: Rat Liver lysates; Lane 2: Rat Urinary bladder lysates; Lane 3: Mouse Stomach lysates; Lane 4: A549 cell lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: A549 cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3: Hela cell lysates; Lane 4: A431 cell lysates ; Lane 5: HepG2 cell lysates;Lane 6: Mouse Stomach lysates; Lane 7: Mouse Lung lysates; Lane 8: Mouse Skeletal muscle (-) lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Pan-Actin Polyclonal Antibody(bs-10196R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse Lung lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Mouse Small intestine lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human A549 cell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Mouse Stomach lysates; Lane 7: Mouse Lung lysates; Lane 8: Mouse Skeletal muscle lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan-Actin) Polyclonal Antibody, Unconjugated (bs-10196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.