bs-10196R-PE [Conjugated Primary Antibody]
Pan-Actin Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Actin

Specificity: Alpha, Beta and Gamma Actins

Immunogen Range: 165-260/377


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 70

Swiss Prot: P68032

Source: KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat
Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han L et al. Interleukin-1β-Induced Senescence Promotes Osteoblastic Transition of Vascular Smooth Muscle Cells. Kidney Blood Press Res. 2020;45(2):314-330. Read more>>
  • Li, Liucheng, et al. "Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats." Phytomedicine 22.1 (2015): 111-119.Read more>>
  • Qiao, Xi, et al. "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction."Nephrology (2015).Read more>>
  • Yan, Xuerui, et al. "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-α-Treated Rat Aortic Smooth Muscle Cells." Cardiovascular Toxicology (2015): 1-9.Read more>>
  • Pang, X-X., et al. "Urotensin II Induces ER Stress and EMT and Increase Extracellular Matrix Production in Renal Tubular Epithelial Cell in Early Diabetic Mice." Kidney and Blood Pressure Research 41.4 (2016): 434-449.Read more>>
  • Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.Read more>>
  • Zhou et al. Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration. (2017) Mol.Med.Re. 15:4123-4131Read more>>
  • Kim et al. Vandetanib and ADAM inhibitors synergistically attenuate the pathological migration of EBV-infected retinal pigment epithelial cells by regulating the VEGF-mediated MAPK pathway. (2017) Exp.Ther.Me. 13:1415-1425Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Liu et al. Therapeutic effects of suppressors of cytokine signaling in diabetic nephropathy. (2014) J.Histochem.Cytoche. 62:119-28Read more>>
  • Gan et al. Preventive effects of ¹²⁵I seeds on benign restenosis following esophageal stent implantation in a dog model. (2015) Mol.Med.Re. 11:3382-90Read more>>
  • Park and Kim Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway. (2018) Mol.Med.Rep. 17:3563-3574Read more>>
  • Ouyang et al. Downregulation of microRNA-429 inhibits cell proliferation by targeting p27Kip1 in human prostate cancer cells. (2015) Mol.Med.Rep. 11:1435-41Read more>>
  • Wang et al. MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2. (2017) Cell.Death.Dis. 8:e2792Read more>>
  • Zeng Y et al. Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.(2018) Lipids in Health and Disease, Nov 6;17(1):251. Read more>>
  • Lu XY et al.Codonopsis tangshen Oliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with Streptozotocin. (2018)Nat Prod Bioprospect.8(6):441-451.Read more>>
  • Jiang L et al. Detecting Vulnerable Atherosclerotic Plaques by 68Ga-Labeled Divalent Cystine Knot Peptide. Mol Pharm. 2019 Mar 4;16(3):1350-1357. Read more>>
  • Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2. Read more>>
  • Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.Read more>>
  • Cai YH et al. Riboflavin/ultraviolet A-induced collagen cross-linking in rabbit corneal scar. Int J Ophthalmol. 2019 Jan 18;12(1):46-50. Read more>>
  • Mao Q et al. Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF‑β1/Smad/miR‑192 signaling pathway. EXPERIMENTAL AND THERAPEUTIC MEDICINE.2019 18: 3053-3061.Read more>>
  • Wu W et al. Yu Jin Pulvis inhibits carbon tetrachloride-induced liver fibrosis by blocking the MAPK and PI3K/Akt signaling pathways. Am J Transl Res. 2019 Sep 15;11(9):5998-6006. eCollection 2019.Read more>>
  • Zhang Y et al. Estrogen facilitates gastric cancer cell proliferation and invasion through promoting the secretion of interleukin-6 by cancer-associated fibroblasts. Int Immunopharmacol. 2019 Nov 18:105937. Read more>>
  • Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.Read more>>
  • Zhao H et al. miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1. Toxicol Appl Pharmacol. 2019 Nov 14;386:114827. Read more>>
  • Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927. Read more>>
  • Hou L et al. Nontoxic concentration of ochratoxin A decreases the dosage of cyclosporine A to induce chronic nephropathy model via autophagy mediated by toll-like receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153. Read more>>
  • Han L et al. Interleukin-1β-Induced Senescence Promotes Osteoblastic Transition of Vascular Smooth Muscle Cells. Kidney Blood Press Res. 2020;45(2):314-330. Read more>>
  • Hou L et al. Nontoxic Concentration of Ochratoxin A Decreases the Dosage of Cyclosporine A to Induce Chronic Nephropathy Model via Autophagy Mediated by Toll-Like Receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153.Read more>>
  • Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-B signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516.Read more>>
  • Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
  • Jing Wang et al. Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD. Mediators Inflamm. 2020 May 8;2020:4260204.Read more>>
  • Wan Q et al. Overexpression of laminin 4 facilitates proliferation and migration of fibroblasts in knee arthrofibrosis by targeting canonical Shh/Gli1 signaling. Connect Tissue Res. 2020 Jun 10;1-11.Read more>>
  • Min Jianget al. P2X7R orchestrates the progression of murine hepatic fibrosis by making a feedback loop from macrophage to hepatic stellate cells. Toxicol Lett. 2020 Oct 15;333:22-32.Read more>>
  • Gong Y et al. Smad3 C-terminal phosphorylation site mutation attenuates the hepatoprotective effect of salvianolic acid B against hepatocarcinogenesisFood Chem Toxicol.2021 Jan;147:111912.Read more>>
  • Chen D et al. Sodium Propionate Attenuates the Lipopolysaccharide-Induced EpithelialCMesenchymal Transition via the PI3K/Akt/mTOR Signaling Pathway. J Agric Food Chem. 2020 Jun 17;68(24):6554-6563.Read more>>
  • Han X et al. The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-beta1/Smad3 pathway. Biomed Pharmacother.2020 Dec 24;134:111135.Read more>>
  • Jianwei Fan. et al. Baicalin Ameliorates Pancreatic Fibrosis by Inhibiting the Activation of Pancreatic Stellate Cells in Mice with Chronic Pancreatitis. Front Pharmacol. 2020; 11: 607133Read more>>
  • Chaoqun Hu. et al. Urine-derived stem cells accelerate the recovery of injured mouse hepatic tissue. Am J Transl Res. 2020; 12(9): 5131C515Read more>>
  • Zheng XP. et al. Kidney-targeted baicalin-lysozyme conjugate ameliorates renal fibrosis in rats with diabetic nephropathy induced by streptozotocin.. Bmc Nephrol. 2020 May;21(1):174-174Read more>>
  • Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-B and TGF-/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275Read more>>
  • Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895C6907Read more>>
  • Wen-qian Jia. et al. CD73 regulates hepatic stellate cells activation and proliferation through Wnt/-catenin signaling pathway. Eur J Pharmacol. 2021 Jan;890:173667Read more>>
  • Qionghua Hu. et al. Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis. J Surg Res. 2021 Sep;265:223Read more>>
  • Xueli Li. et al. Dynamic expression of vascular endothelial growth factor (VEGF) and platelet-derived growth factor receptor beta (PDGFR) in diabetic brain contributes to cognitive dysfunction. Brain Res Bull. 2021 Oct;175:99Read more>>
  • Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634Read more>>
  • Xiaohui Chen. et al. Eleutheroside B-loaded poly (lactic-co-glycolic acid) nanoparticles protect against renal fibrosis via Smad3-dependent mechanism. 2021 Sep 28Read more>>
  • Ms. Nannan Zhang. et al. Characterization of Urine Derived Stem Cells from Patients with End-Stage Liver Diseases and Application to Induced Acute and Chronic Liver Injury of Nude Mice Model. 2021 Sep 22Read more>>
  • Hong Zhu. et al. Sennoside A alleviates inflammatory responses by inhibiting the hypermethylation of SOCS1 in CCl4-induced liver fibrosis. Pharmacol Res. 2021 Oct;:105926Read more>>
  • Han Ding. et al. JQ-1 ameliorates schistosomiasis liver fibrosis by suppressing JAK2 and STAT3 activation. Biomed Pharmacother. 2021 Dec;144:112281Read more>>
VALIDATION IMAGES