bs-1019R [Primary Antibody]
TLR2 Polyclonal Antibody
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Host: Rabbit

Target Protein: TLR2

Immunogen Range: 701-784/784


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7097

Swiss Prot: O60603

Source: KLH conjugated synthetic peptide derived from human TLR2 (cytoplasmic domain)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 84


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Takanashi, Naoya, et al. "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." BMC Microbiology 13.1 (2013): 54.Read more>>
  • Tomofuji, Takaaki, et al. "Involvement of toll-like receptor 2 and 4 in association between dyslipidemia and osteoclast differentiation in apolipoprotein E deficient rat periodontium." Lipids in Health and Disease 12.1 (2013): 1.Read more>>
  • Tang, Lu, et al. "Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts." Archives of Oral Biology 56.10 (2011): 1064-1072.Read more>>
  • Chen, Xiaoming, et al. ʺSargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors.ʺ Carbohydrate Polymers (2014).Read more>>
  • Tan, Yan, et al. "Stiffening-Induced High Pulsatility Flow Activates Endothelial Inflammation via a TLR2/NF-κB Pathway." PLoS one 9.7 (2014).Read more>>
  • Mannic, Tiphaine, et al. "CD14 as a mediator of the Mineralocorticoid Receptor-dependent anti-apolipoproteinA-1 IgG chronotropic effect on cardiomyocytes."Endocrinology (2015): en-2015.Read more>>
  • Zhu, Ya‑Jun, et al. "Toll-like receptor-2 and-4 are associated with hyperlipidemia." Molecular Medicine Reports.Read more>>
  • Hardigan, Trevor, Yasir Abdul, and Adviye Ergul. "Linagliptin reduces effects of ET-1 and TLR2-mediated cerebrovascular hyperreactivity in diabetes." Life Sciences (2016).Read more>>
  • Schimunek, Lukas, et al. "Early decreased TLR2 expression on monocytes is associated with their reduced phagocytic activity and impaired maturation in a porcine polytrauma model." PloS one 12.11 (2017): e0187404.Read more>>
  • Xu, Wei, et al. "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson’s Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." Cellular Physiology and Biochemistry 44.6 (2017): 2091-2104.Read more>>
  • Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302Read more>>
  • Yang et al. Sialic acid rescues repurified lipopolysaccharide-induced acute renal failure via inhibiting TLR4/PKC/gp91-mediated endoplasmic reticulum stress, apoptosis, autophagy, and pyroptosis signaling. (2014) Toxicol.Sci. 141:155-65Read more>>
  • Barreiros et al. Immunohistochemical and mRNA expression of RANK, RANKL, OPG, TLR2 and MyD88 during apical periodontitis progression in mice. (2018) J.Appl.Oral.Sci. 26:e20170512Read more>>
  • Mödinger, Y.et al. C5aR1 interacts with TLR2 in osteoblasts and stimulates the osteoclast-inducing chemokine CXCL10. J Cell Mol Med. 2018 Sep 24.Read more>>
  • Zhang J et al. MiR-146a promotes oligodendrocyte progenitor cell differentiation and enhances remyelination in a model of experimental autoimmune encephalomyelitis. Neurobiol Dis. 2019 May;125:154-162. Read more>>
  • Dong W et al. Polychlorinated biphenyl quinone induces caspase 1-mediated pyroptosis through the induction of pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD signal axis. Chem Res Toxicol. 2019 Apr 23. Read more>>
  • Li T et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis. R. Soc. open sci. 2019,6: 181604.Read more>>
  • Li T et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis.R Soc Open Sci. 2019 Jul 3;6(7):181604. Read more>>
  • Zhan Q et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.Read more>>
  • Houpan Song et al. Traditional Chinese Medicine Li-Zhong-Tang accelerates the healing of indomethacin-induced gastric ulcers in rats by affecting TLR-2/MyD88 signaling pathway. J Ethnopharmacol. 2020 Sep 15;259:112979.Read more>>
  • Zhao Yonglin. et al. Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation through a Toll-like Receptor-Related Pathway after Diffuse Axonal Injury in Rats. Biomed Res Int. 2020;2020:5946205Read more>>
  • Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:12405Read more>>
  • Xuelian Ma. et al. Down-regulated long non-coding RNA RMST ameliorates dopaminergic neuron damage in Parkinsons disease rats via regulation of TLR/NF-B signaling pathway. Brain Res Bull. 2021 ApRead more>>
  • Fang Jia. et al. Cytotoxicity and anti-inflammatory effect of a novel diminazene aceturate derivative in bovine mammary epithelial cells. Res Vet Sci. 2021 Jul;137:102Read more>>
  • Rossetto Isabela Maria Urra. et al. Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum. Toxicol Res-Uk. 2021 Aug;10(4):835-849Read more>>
  • Jing Zhang. et al. Exosomes derived from bone marrow mesenchymal stromal cells promote remyelination and reduce neuroinflammation in the demyelinating central nervous system. Exp Neurol. 2022 Jan;347:113895Read more>>
  • Yao Zheng. et al. Comparative proteomic analysis of spleen reveals key immune-related proteins in the yak (Bos grunniens) at different growth stages. Comp Biochem Phys D. 2022 Jun;42:100968Read more>>
  • I.B. Reis. et al. OncoTherad (MRB-CFI-1) nano-immunotherapy reduced tumoral progression in non-muscle invasive bladder cancer through activation of Toll-like signaling pathway. Tissue Cell. 2022 Jun;76:101762Read more>>
  • Zhang, Peitao. et al. Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors. Nat Commun. 2022 Mar;13(1):1-18Read more>>
  • Qi Wang. et al. Effect of oral administration of Limosilactobacillus reuteri on intestinal barrier function and mucosal immunity of suckling piglets. Ital J Anim Sci. 2022;21(1):612-623Read more>>
  • Canan Ero?lu Gne?. et al. Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells. GENE. 2022 Aug;:146805Read more>>
  • Xu Bai. et al. Toll-like Receptor 2 Is Associated with the Immune Response, Apoptosis, and Angiogenesis in the Mammary Glands of Dairy Cows with Clinical Mastitis. INT J MOL SCI. 2022 Jan;23(18):10717Read more>>
  • Xu Bai. et al. Toll-like Receptor 2 Is Associated with the Immune Response, Apoptosis, and Angiogenesis in the Mammary Glands of Dairy Cows with Clinical Mastitis. INT J MOL SCI. 2022 Jan;23(18):10717Read more>>
  • Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
  • Lihui Ni. et al. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. INT J BIOL MACROMOL. 2023 Jul;242:12475Read more>>
  • Mao Yu-Zhi. et al. Pudilan Anti-inflammatory Oral Liquid and Organic Acid Component from Taraxaci Herba Attenuate Allergic Asthma in Young Mice through Toll-like Receptor 2/Toll-like Receptor 4 Signaling Pathway. World Journal of Traditional Chinese Medicine. 2024 Apr;:10.4103/wjtcm.wjtcm_64_24Read more>>
  • Gan Cao. et al. Total Alkaloids of Sophora alopecuroides Linn. Attenuates Rheumatoid Arthritis Through Regulating Follicular Helper T Cells. J INFLAMM RES. 2024 03Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse spleen cells probed with \tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Human A549 cells probed with Rabbit Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).


Lane 1: Mouse Spleen; Lane 2: Mouse Thymus; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Raw264.7 Cells; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.


Lane 1: Cerebrum lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Rat Spleen lysates; Lane 2: Rat Bronchus lysates; Lane 3: Rat Lung lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-TLR2 antibody (bs-1019R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.


The Raw264.7 (M) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-TLR2 antibody (bs-1019R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.