Host: Rabbit
Target Protein: Sclerostin
Immunogen Range: 121-180/213
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 50964
Swiss Prot: Q9BQB4
Source: KLH conjugated synthetic peptide derived from human Sclerostin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 21
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Diao et al. The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact. (2017) Sci.Rep. 7:6508Read more>>
- Surowiec RK et al. A Xenograft Model to Evaluate the Bone Forming Effects of Sclerostin Antibody in Human Bone Derived from Pediatric Osteogenesis Imperfecta Patients. Bone. 2020 Jan;130:115118.Read more>>
- Zhang C et al. FOXO1 Mediates Advanced Glycation End Products Induced Mouse Osteocyte-Like MLO-Y4 Cell Apoptosis and Dysfunctions. Journal of Diabetes Research.2019, Article ID 6757428.Read more>>
- Yiqi Yanget al. Bioprinting of an osteocyte network for biomimetic mineralization. Biofabrication. 2020 Jul 29;12(4):045013.Read more>>
- Yoo-Sung Nam. et al. Sclerostin in Periodontal Ligament: Homeostatic Regulator in Biophysical Force-Induced Tooth Movement. J CLIN PERIODONTOL. 2022 Apr 03Read more>>
- Young Jae Moon. et al. Sirtuin 6 Overexpression Improves Rotator Cuff Tendon-to-Bone Healing in the Aged. CELLS-BASEL. 2023 Jan;12(16):2035Read more>>
- Chen Shengqiang. et al. Local H2 release remodels senescence microenvironment for improved repair of injured bone. NAT COMMUN. 2023 Nov;14(1):1-16Read more>>
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-Sclerostin Polyclonal Antibody, Unconjugated(bs-10200R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sclerostin) Polyclonal Antibody, Unconjugated (bs-10200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sclerostin) Polyclonal Antibody, Unconjugated (bs-10200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.