bs-10217R [Primary Antibody]
ADORA2B Polyclonal Antibody
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Host: Rabbit

Target Protein: ADORA2B

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11140, 135

Swiss Prot: P29274

Source: KLH conjugated synthetic peptide derived from human ADORA2B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ADORA2B Polyclonal Antibody, Unconjugated (bs-10217R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


SW480 lysates probed with Rabbit Anti-ADORA2B Polyclonal Antibody, Unconjugated (bs-10217R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADORA2 Polyclonal Antibody, Unconjugated (bs-10217R) at 1:200 overnight at 4°C, DAB staining.