bs-1021R
[Primary Antibody]
TLR4 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TLR4
Immunogen Range: 751-835/835
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 21898
Swiss Prot: Q9QX05
Source: KLH conjugated synthetic peptide derived from rat TLR4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 90
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Cow
Predicted Cross Reactive Species:
Dog
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Takanashi, Naoya, et al. "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." BMC Microbiology 13.1 (2013): 54.Read more>>
- Nakayama Y et al. Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl Immunol. 2020 Feb;58:101263.Read more>>
- Tang, Lu, et al. "Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts." Archives of Oral Biology 56.10 (2011): 1064-1072.Read more>>
- Chen, Xiaoming, et al. ʺSargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors.ʺ Carbohydrate Polymers (2014).Read more>>
- Xing, Shanshan, et al. "Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis." Journal of International Medical Research (2014): 0300060514534645.Read more>>
- Wang, Dunjing, et al. "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-κB Activation in Microglial Cells." Inflammation: 1-8.Read more>>
- Namisaki, Tadashi, et al. "Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis." Journal of Gastroenterology (2015): 1-11.Read more>>
- ARFIAN, NUR, et al. "Vitamin D Attenuates Kidney Fibrosis via Reducing Fibroblast Expansion, Inflammation, and Epithelial Cell Apoptosis." Kobe J. Med. Sci 62.2 (2016): E38-E44.Read more>>
- Kawakita, Fumihiro, et al. "Effects of Toll-Like Receptor 4 Antagonists Against Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage in Mice." Molecular Neurobiology (2016): 1-10.Read more>>
- Harasymowicz, Natalia S., et al. "Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA." Arthritis & Rheumatology (2017).Read more>>
- Jenvey, Caitlin J., and Judith R. Stabel. "Sudan Black B masks Mycobacterium avium subspecies paratuberculosis immunofluorescent antibody labeling." Journal of Histology & Histopathology 4.1 (2017): 11.Read more>>
- Kulhankova, Katarina, et al. "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." Infection and immunity (2017): IAI-00848.Read more>>
- Xu, Wei, et al. "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson’s Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." Cellular Physiology and Biochemistry 44.6 (2017): 2091-2104.Read more>>
- Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302Read more>>
- Qiao et al. Effect of different 1, 25-(OH)2D3 doses on high mobility group box1 and toll-like receptors 4 expression in lung tissue of asthmatic mice. (2015) Int.J.Clin.Exp.Med. 8:4016-23Read more>>
- Zhao et al. Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats. (2016) Mediators.Inflamm. 2016:4706915Read more>>
- Li C et al. UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells. Oxid Med Cell Longev. 2019 Feb 10;2019:6505373. Read more>>
- Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).Read more>>
- Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
- Zhang CY et al. Simvastatin alleviates inflammation and oxidative stress in rats with cerebral hemorrhage through Nrf2-ARE signaling pathway. Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6321-6329. Read more>>
- Zhan Q et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.Read more>>
- Li Z et al. Methane-Rich Saline Counteracts Cholestasis-Induced Liver Damage via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway. Oxidative Medicine and Cellular Longevity, 2019, 1–13.Read more>>
- Winter J et al. Adenine Nucleotide Translocase 1 Expression is Coupled to the HSP27-Mediated TLR4 Signaling in Cardiomyocytes. Cells. 2019 Dec 6;8(12). pii: E1588.Read more>>
- Nakayama Y et al. Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl Immunol. 2020 Feb;58:101263.Read more>>
- Peng L et al. Madecassoside Protects Against LPS-Induced Acute Lung Injury via Inhibiting TLR4/NF-B Activation and Blood-Air Barrier Permeability. Front Pharmacol. 2020 Jun 5;11:807.Read more>>
- Cheng Z et al. Cotinine inhibits TLR4/NF-B signaling pathway and improves deep vein thrombosis in rats. Biosci Rep. 2020 Jun 26;40(6):BSR20201293.Read more>>
- Chen H et al. Sevoflurane attenuates cognitive dysfunction and NLRP3-dependent caspase-1/11-GSDMD pathway-mediated pyroptosis in the hippocampus via upregulation of SIRT1 in a sepsis model. Arch Physiol Biochem. 2020 Jun 13;1-8.Read more>>
- Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
- Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441Read more>>
- Shun Huang. et al. Design, Synthesis, and Activity Study of Cinnamic Acid Derivatives as Potent Antineuroinflammatory Agents. Acs Chem Neurosci. 2021;12(3):419C429Read more>>
- Yuhua Li. et al. Jiaolong capsule protects SD rats against 2,4,6-trinitrobenzene sulfonic acid induced colitis. J Ethnopharmacol. 2021 Apr;269:113716Read more>>
- Jiajia Wang. et al. TAK-242 ameliorates DSS-induced colitis by regulating the gut microbiota and the JAK2/STAT3 signaling pathway. Microb Cell Fact. 2020 Dec;19(1):1-17Read more>>
- Lina D. Eissa. et al. Inhibition of thioredoxin-interacting protein and inflammasome assembly using verapamil mitigates diabetic retinopathy and pancreatic injury. Eur J Pharmacol. 2021 Mar;:174061Read more>>
- Lili Li. et al. Protein overexpression of toll?like receptor 4 and myeloid differentiation factor 88 in oral squamous cell carcinoma and clinical significance. Oncol Lett. 2021 Nov;22(5):1-11Read more>>
- Sun, Lizhong. et al. Fibroblast membrane-camouflaged nanoparticles for inflammation treatment in the early stage. Int J Oral Sci. 2021 Nov;13(1):1-14Read more>>
- Aykut Ozturk. et al. The protective effects of trimetazidine against ovary ischemiareperfusion injury via the TLR4/Nf-kB signal pathway. J BIOCHEM MOL TOXIC. 2022 May 27Read more>>
- Mona F. El-Azab. et al. A novel role of Nano selenium and sildenafil on streptozotocin-induced diabetic nephropathy in rats by modulation of inflammatory, oxidative, and apoptotic pathways. LIFE SCI. 2022 Aug;303:120691Read more>>
- Chunyan Lei. et al. HMGB1/TLR4 induces autophagy and promotes neuroinflammation after intracerebral hemorrhage. BRAIN RES. 2022 Jul;:148003Read more>>
- Irene Palenca. et al. N-Palmitoyl-D-Glucosamine Inhibits TLR-4/NLRP3 and Improves DNBS-Induced Colon Inflammation through a PPAR-α-Dependent Mechanism. BIOMOLECULES. 2022 Aug;12(8):1163Read more>>
- Canan Ero?lu Gne?. et al. Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells. GENE. 2022 Aug;:146805Read more>>
- Ruiyue Sun. et al. Effects of Bacillus subtilis natto JLCC513 on gut microbiota and intestinal barrier function in obese rats. J APPL MICROBIOL. 2022 SepRead more>>
- Jingwen Liu. et al. The effects of Saccharomyces boulardii on rat colonic hypermotility induced by repeated water avoidance stress and the potential mechanism. PEERJ. 2022 Nov;10:e1439Read more>>
- aoming Tian. et al. Modulating effects of Hericium erinaceus polysaccharides on the immune response by regulating gut microbiota in cyclophosphamide-treated mice. J SCI FOOD AGR. 2022 DecRead more>>
- Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
- Siyu Qiang. et al. Changes in the content of Puerarin-PLGA nanoparticles in mice under the influence of alcohol and analysis of their antialcoholism. J APPL BIOMATER FUNC. ;()Read more>>
- aoming Tian. et al. Ameliorating effects of Hericium erinaceus polysaccharides on intestinal barrier injury in immunocompromised mice induced by cyclophosphamide. FOOD FUNCT. 2023 FebRead more>>
- Z. Chen. et al. Mechanism of Resveratrol Improving Rheumatoid Arthritis Injury by Inhibiting S100A8/A9 Expression. INDIAN J PHARM SCI. 2023 Feb;85(1):145-151Read more>>
- Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
- Xiahong Tang. et al. Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 MayRead more>>
- Yixuan Liang. et al. Dihydrotestosterone mediates the inflammation effect under lipopolysaccharides in bovine endometrial epithelial cells via AR blockading TLR4/MyD88 signaling pathway. ANIM REPROD SCI. 2023 Aug;255:107292Read more>>
VALIDATION IMAGES
Image submitted by One World Lab validation program. HL60 and MCF-7 cells were stained with rabbit polyclonal antibody against TLR4 with two dilutions (1:100 and 1:250). 2nd antibody without primary antibody was used as control included here.
Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse macrophages(RAW264.7)labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TLR4 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 overnight at 4°C, DAB staining.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TLR4 Polyclonal Antibody(bs-1021R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human HL-60 cell lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.