bs-1023R [Primary Antibody]
CSF3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CSF3

Immunogen Range: 155-198/207


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1440

Swiss Prot: P09919

Source: KLH conjugated synthetic peptide derived from human CSF3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 19


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kameyama, Hiroshi, et al. "The mRNA Expressions and Immunohistochemistry of Factors Involved in Angiogenesis and Lymphangiogenesis in the Early Stage of Rat Skin Incision Wounds." Legal Medicine (2015).Read more>>
  • Volkmann J et al. Kidney injury enhances renal G‐CSF expression and modulates granulopoiesis and human neutrophil CD177 in vivo. Clin Exp Immunol. 2019 Sep 11. Read more>>
  • Hong L et al. Protective effects of Human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage. J Mol Cell Biol. 2020 Jun 11;12(5):372-385.Read more>>
  • Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110. Read more>>
  • Wen-Chieh Liao. et al. CHPF promotes malignancy of breast cancer cells by modifying syndecan-4 and the tumor microenvironment. Am J Cancer Res. 2021; 11(3): 812C826Read more>>
  • Yi Yang. et al. T/Interleukin-17A Contributes to the Effect of Maresin Conjugates in Tissue Regeneration 1 on Lipopolysaccharide-Induced Cardiac Injury. Front Immunol. 2021; 12: 674542Read more>>
  • Ting Shang. et al. The combination of four main components in Xuebijing injection improved the preventive effects of Cyclosporin A in acute graft-versus-host disease mice by protecting intestinal microenvironment. Biomed Pharmacother. 2022 Apr;148:112675Read more>>
VALIDATION IMAGES

L1 rat brain, L2 rat heart lysates probed (bs-1023R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 19kDa


"Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-CSF3/G-CSF Polyclonal Antibody (bs-1023R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining\n"


Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.