bs-10249R [Primary Antibody]
MCT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MCT1

Immunogen Range: 251-350/500


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6566

Swiss Prot: P53985

Source: KLH conjugated synthetic peptide derived from human MCT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 55


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Prado-Garcia H et al. Lactic Acidosis in the Presence of Glucose Diminishes Warburg Effect in Lung Adenocarcinoma Cells. Front Oncol. 2020 Jun 12;10:807.Read more>>
  • Carolina Manosalva. et al. D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFB Pathways. Animals-Basel. 2020 Nov;10(11):2105Read more>>
  • Li Huihui. et al. Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR . Front Pharmacol. 2020 Aug;11:1255Read more>>
  • Makoto Nogami. et al. Hyperketonemia as the diagnostic basis for hypothermia: An experimental study in diabetic and control mice. Legal Med-Tokyo. 2021 Sep;52:101908Read more>>
  • Wang Shijun. et al. Investigations on the Changes of Serum Proteins in Rabbits after Trimeresurus stejnegeri Venom Injection via Mass Spectrometry-Based Proteomics. EVID-BASED COMPL ALT. 2022;2022:9239662Read more>>
  • P.P.M. Scariot. et al. Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training. LIFE SCI. 2022 Oct;307:120872Read more>>
  • Pedro Paulo Menezes Scariot. et al. Living high - training low model applied to C57BL/6J mice: Effects on physiological parameters related to aerobic fitness and acid-base balance. LIFE SCI. 2023 Mar;317:121443Read more>>
  • Stollmeier, Martin. et al. AirCliquid interface cultures trigger a metabolic shift in intestinal epithelial cells (IPEC-1). HISTOCHEM CELL BIOL. 2023 Feb;:1-12Read more>>
VALIDATION IMAGES

Lane 1: A431 lysates; probed with MCT1 Polyclonal Antibody, Unconjugated (bs-10249R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.


K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MCT1 Polyclonal Antibody(bs-10249R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).