bs-10257R [Primary Antibody]
COX4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: COX4

Immunogen Range: 51-169/169


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1327

Swiss Prot: P13073

Source: KLH conjugated synthetic peptide derived from human COX4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 17


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Da-Ting, et al. "Artemisinin mimics calorie restriction to trigger mitochondrial biogenesis and compromise telomere shortening in mice." PeerJ 3 (2015): e822.Read more>>
  • Wu, Bin, et al. "Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2017).Read more>>
  • Gao, Jinhang, et al. "De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer." Molecular medicine reports 12.5 (2015): 7033-7038.Read more>>
  • Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
  • Wang Y et al.Fine particulate matter induces mitochondrial dysfunction and oxidative stress in human SH-SY5Y cells.(2018) Chemosphere;218:577-588. Read more>>
  • Zhang JT et al. CRISPR/Cas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFα/CHX-induced Apoptosis. BioMed Research International.2019, Article ID 9071297.Read more>>
  • Yue Wang # et al. Mitophagy Induced by Mitochondrial Function Damage in Chicken Kidney Exposed to Cr(VI). Biol Trace Elem Res. 2021 Feb;199(2):703-711.Read more>>
VALIDATION IMAGES

Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated,bs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


HeLa cells probed with Rabbit Anti-COX4 Polyclonal Antibody (bs-R) at 1:20 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG conjugated secondary FITC (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\n


Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Mouse kidney lysates probed with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.