bs-1025R [Primary Antibody]
HGF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HGF

Immunogen Range: 201-300/723


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3082

Swiss Prot: P14210

Source: KLH conjugated synthetic peptide derived from human HGF alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54/80


Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yan B et al. Paracrine HGF/c-MET enhances the stem cell-like potential and glycolysis of pancreatic cancer cells via activation of YAP/HIF-1α.Exp Cell Res. 2018 Oct 1;371(1):63-71.Read more>>
  • Yu, Xiuju, et al. "Expression and tissue distribution of hepatocyte growth factor (HGF) and its receptor (c-Met) in alpacas (Vicugna pacos) skins associated with white and brown coat colors." Acta Histochemica (2015).Read more>>
  • Li, Hongyu, et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer." Oncology letters9.5 (2015): 1989-1996.Read more>>
  • Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016).Read more>>
  • Wu, Ka, et al. "Biocharacterization of Heat Shock Protein 90 in Acetaminophen-Treated Livers Without Conspicuous Drug Induced Liver Injury." Cellular Physiology and Biochemistry 43.4 (2017): 1-1.Read more>>
  • Zhi et al. Effects of PHA-665752 and vemurafenib combination treatment onin vitroand murine xenograft growth of human colorectal cancer cells with BRAFV600Emutations. (2018) Oncol.Lett. 15:3904-3910Read more>>
  • Xu X et al. Potential biomarker of fibroblast growth factor 21 in valproic acid-treated livers. Biofactors. 2019 May 23. Read more>>
  • Yan B et al. Paracrine HGF/c-MET enhances the stem cell-like potential and glycolysis of pancreatic cancer cells via activation of YAP/HIF-1α.Exp Cell Res. 2018 Oct 1;371(1):63-71.Read more>>
  • Quanyu Chen. et al. Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. Int Immunopharmacol. 2021 Jan;90:10718Read more>>
  • Yan, Liting. et al. HGF can reduce accumulation of inflammation and regulate glucose homeostasis in T2D mice. 2021 Aug 07Read more>>
  • Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;()Read more>>
  • Liu, Jingyu. et al. Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder. Stem Cell Res Ther. 2022 Dec;13(1):1-16Read more>>
  • Kobayashi, Tsukasa. et al. Prenatal glucocorticoid administration accelerates the maturation of fetal rat hepatocytes. Mol Biol Rep. 2022 Mar;:1-12Read more>>
  • Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-HGF Polyclonal Antibody, Unconjugated(bs-1025R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HGF Polyclonal Antibody, Unconjugated (bs-1025R) at 1:400 overnight at 4°C, DAB staining.