Host: Rabbit
Target Protein: NSE
Immunogen Range: 201-300/434
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2026
Swiss Prot: P09104
Source: KLH conjugated synthetic peptide derived from human NSE
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
Dog
Others
Predicted Cross Reactive Species:
Cow
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
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- Huang, Xiaopeng, et al. "A novel reverse fluorescent immunoassay approach for sensing human chorionic gonadotropin based on silver-gold nano-alloy and magnetic nanoparticles." Analytical and bioanalytical chemistry (2015): 1-9.Read more>>
- Yan et al. Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage. (2017) Iran.J.Basic.Med.Sc. 20:1029-1036Read more>>
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- Zheng X et al. Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots. Mikrochim Acta. 2019 Nov 20;186(12):817. Read more>>
- Li, Jun. et al. Immunoproteasome inhibition prevents progression of castration-resistant prostate cancer. BRIT J CANCER. 2023 Jan;:1-14Read more>>
Neuroblastoma cells labeled with Anti-NSE/ENO2/γ Enolase Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200, followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-NSE/ENO2/γ Enolase Polyclonal Antibody, Unconjugated (bs-1027R) at 1:800, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
A431 lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse brain lysates; Lane 2: Mouse cerebellum lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spinal cord lysates; Lane 2: Rat Spinal cord lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200 overnight at 4°C, DAB staining.