bs-10362R [Primary Antibody]
MPL Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MPL

Immunogen Range: 401-500/635


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4352

Swiss Prot: P40238

Source: KLH conjugated synthetic peptide derived from human MPL

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Maekawa, T., et al. "Myeloproliferative leukemia protein activation directly induces fibrocyte differentiation to cause myelofibrosis." Leukemia (2017).Read more>>
  • Xin Chen. et al. Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production. Theranostics. 2021; 11(10): 4655C4671Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat spleen tissue labeled with Rabbit Anti-MPL Polyclonal Antibody, Unconjugated (bs-10362R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPL Polyclonal Antibody(bs-10362R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).