Host: Rabbit
Target Protein: LPA2
Immunogen Range: 1-100/351
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9170
Swiss Prot: Q9HBW0
Source: KLH conjugated synthetic peptide derived from human LPA2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 39
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Mizuki Uenaka. et al. LPA2 promotes neuronal differentiation and neurite formation in neocortical development. Biochem Bioph Res Co. 2022 Apr;598:89Read more>>
- Xue-min ZHANG. et al. Effects of LPA on the development of sheep in vitro fertilized embryos and attempt to establish sheep embryonic stem cells. J INTEGR AGR. 2022 AugRead more>>
- Runan Zhang. et al. A mutation in LPAR2 activates the miR-939-5p-LPAR2-PI3K/AKT axis to regulate the proliferation and apoptosis of granulosa cells in sheep. THERIOGENOLOGY. 2024 Apr;219:1Read more>>
Mouse splenocytes probed with \tRabbit Anti-LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary \n(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control orange).
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-LPA2 Polyclonal Antibody, Unconjugated (bs-10368R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LPA2) polyclonal Antibody, Unconjugated (bs-10368R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA2) Polyclonal Antibody, Unconjugated (bs-10368R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.