bs-1036R [Primary Antibody]
CD62L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD62L

Immunogen Range: 301-372/372


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20343

Swiss Prot: P18337

Source: KLH conjugated synthetic peptide derived from mouse CD62L

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 37


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Vries, Mark HM, et al. "CXCL1 promotes arteriogenesis through enhanced monocyte recruitment into the peri-collateral space." Angiogenesis (2014): 1-9.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Rabbit Anti-L-Selectin/CD62L Polyclonal Antibody (bs-1036R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Rabbit Anti-L-Selectin/CD62L Polyclonal Antibody (bs-1036R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat colitis tissue labeled with Rabbit Anti-L-Selectin/CD62L Polyclonal Antibody (bs-1036R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 rat heart lysates L2 rat brain lysates probed with Anti L-Selectin/CD62L Polyclonal Antibody, Unconjugated (bs-1036R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 38kD. Observed band size:38kD.\n


Mouse splenocytes probed with CD62L Polyclonal Antibody, Unconjugated (bs-1036R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).