bs-10412R
[Primary Antibody]
VEGFR2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: VEGFR2
Immunogen Range: 101-200/1356
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3791
Swiss Prot: P35968
Source: KLH conjugated synthetic peptide derived from human VEGFR2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 147
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ahmad S et al. C3a Receptor Antagonist Therapy is Protective with or without Thrombolysis in Murine Thromboembolic Stroke. Br J Pharmacol. 2020 Jan 24.Read more>>
- Fernández, RA García, et al. "Iberian pig early pregnancy: Vascular endothelial growth factor receptor system expression in the maternofetal interface in healthy and arresting conceptuses." Theriogenology 83.3 (2015): 334-343.Read more>>
- Tan, Jie, et al. "Single-Dose Local Simvastatin Injection Improves Implant Fixation via Increased Angiogenesis and Bone Formation in an Ovariectomized Rat Model." Medical Science Monitor 21 (2015): 1428-1439.Read more>>
- Wang, Li, et al. "Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model." Peptides (2017).Read more>>
- Xiao et al. Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model. (2017) Oncotarget. 8:86471-86487Read more>>
- Homan KA et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro. Nat Methods. 2019 Mar;16(3):255-262.Read more>>
- Bhagwani AR et al. Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension.PLoS One. 2019 Mar 18;14(3):e0213890.Read more>>
- Zhao F et al. Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation. Tissue Eng Part A. 2019 Jun 26.Read more>>
- Luo Z et al. Silencing of RBP‑JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Mol Med Rep. 2019 Nov 6.Read more>>
- Ahmad S et al. C3a Receptor Antagonist Therapy is Protective with or without Thrombolysis in Murine Thromboembolic Stroke. Br J Pharmacol. 2020 Jan 24.Read more>>
- Ding J et al. Anti-angiogenic effect of a chemically sulfated polysaccharide from Phellinus ribis by inhibiting VEGF/VEGFR pathway. Int J Biol Macromol. 2020 Mar 11;154:72-81. Read more>>
- Hongxia Wei. et al. MRI tracing of ultrasmall superparamagnetic iron oxide nanoparticle?labeled endothelial progenitor cells for repairing atherosclerotic vessels in rabbits. Mol Med Rep. 2020 Oct;22(4):3327-3337Read more>>
- Kuo Zhang. et al. A Monotargeting Peptidic Network Antibody Inhibits More Receptors for Anti-Angiogenesis. Acs Nano. 2021;XXXX(XXX):XXX-XXXRead more>>
- Tu Yi-An. et al. Intentional endometrial injury enhances angiogenesis through increased production and activation of MMP-9 by TNF- and MMP-3 in a mouse model. Mol Hum Reprod. 2021 AugRead more>>
- Wu Yefeng. et al. CircLATS2 Regulates miR-520a-3p/E2F7/p-VEGFR2 Signaling Pathway to Promote Hepatocellular Carcinoma Progression and Angiogenesis. J ONCOL. J Oncol. 2022;2022:374456Read more>>
- Yongxin Guo. et al. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. INT J MOL SCI. 2022 Jan;23(21):13152Read more>>
- Taha, Amna Mohamed. et al. Ramucirumab combination with sorafenib enhances the inhibitory effect of sorafenib on HepG2 cancer cells. SCI REP-UK. 2022 Oct;12(1):1-16Read more>>
- Maria Consiglia Trotta. et al. Oral Administration of Vitamin D3 Prevents Corneal Damage in a Knock-Out Mouse Model of Sjögren’s Syndrome. BIOMEDICINES. 2023 Feb;11(2):616Read more>>
- Di Wu. et al. Immunomagnetic Delivery of Adipose-Derived Endothelial Progenitor Cells for the Repair of Renal Ischemia–Reperfusion Injury in a Rat Model. Bioengineering-Basel. 2023 May;10(5):509Read more>>
- Adam Mitchell. et al. Rapid Generation of Pulmonary Organoids from Induced Pluripotent Stem Cells by Co-Culturing Endodermal and Mesodermal Progenitors for Pulmonary Disease Modelling. BIOMEDICINES. 2023 May;11(5):1476Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse placenta tissue labeled with Anti-VEGFR2 Polyclonal Antibody, Unconjugated (bs-10412R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HeLa cells probed with Rabbit Anti-VEGFR2 Polyclonal Antibody (bs-10412R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.