bs-10419R [Primary Antibody]
GPI Polyclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: GPI

Immunogen Range: 401-500/558


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51701

Source: KLH conjugated synthetic peptide derived from human Glucose 6 phosphate isomerase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 63


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shao Fangyu. et al. B cell-derived anti-beta 2 glycoprotein I antibody contributes to hyperhomocysteinaemia-aggravated abdominal aortic aneurysm. Cardiovasc Res. 2020 Sep;116(11):1897-1909Read more>>
VALIDATION IMAGES

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Esophagus tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Esophagus tissue lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human U251 cell lysates; Lane 8: Human HepG2 cell lysates probed with GPI Polyclonal Antibody, Unconjugated (bs-10419R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.