bs-10423R [Primary Antibody]
Collagen I Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: Collagen I

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1277

Swiss Prot: P02452

Source: KLH conjugated synthetic peptide derived from human Collagen I

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Predicted Molecular Weight: 130


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. Read more>>
  • Hu, Jianguo, Biao Zeng, and Xingwei Jiang. "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious."International journal of clinical and experimental pathology 8.2 (2015): 1525.Read more>>
  • Zhou, Nan, et al. "Imperatorin derivative OW1 inhibits the upregulation of TGF-β and MMP-2 in renovascular hypertension-induced cardiac remodeling." Experimental and therapeutic medicine 11.5 (2016): 1748-1754.Read more>>
  • Zhu, Yi, et al. "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." Medical Science Monitor 22 (2016): 3438-3445.Read more>>
  • Jiang, Ke, et al. "Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes." Molecular medicine reports 13.4 (2016): 3567-3573.Read more>>
  • Li, Yin, Lin Xiong, and Jianping Gong. "Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells." American Journal of Translational Research 9.6 (2017): 2865-2877.Read more>>
  • Miyauchi, Yuya, et al. "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." Scientific Reports 7 (2017).Read more>>
  • Belair, David G., et al. "Engineering human cell spheroids to model embryonic tissue fusion in vitro." PloS one 12.9 (2017): e0184155.Read more>>
  • Velázquez, Kandy T., et al. "miR155 deficiency aggravates high‐fat diet‐induced adipose tissue fibrosis in male mice." Physiological Reports 5.18 (2017): e13412.Read more>>
  • Chiang et al. R1R2 peptide ameliorates pulmonary fibrosis in mice through fibrocyte migration and differentiation. (2017) PLoS.On. 12:e0185811Read more>>
  • Liang et al. A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix. (2016) Sci.Re. 6:18205Read more>>
  • Lu et al. A novel silk fibroin nanofibrous membrane for guided bone regeneration: a study in rat calvarial defects. (2016) Am.J.Transl.Res. 7:2244-53Read more>>
  • Li et al. Morphological effects of MMPs inhibitors on the dentin bonding. (2015) Int.J.Clin.Exp.Med. 8:10793-803Read more>>
  • Du et al. MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a. (2015) Sci.Rep. 5:16163Read more>>
  • Pan et al. DNA Methylation of PTGIS Enhances Hepatic Stellate Cells Activation and Liver Fibrogenesis. (2018) Front.Pharmacol. 9:553Read more>>
  • Li,et al.Effects of sub-chronic exposure to atmospheric PM2.5 on fibrosis, inflammation, endoplasmic reticulum stress and apoptosis in the livers of rats.(2018) Toxicology Research. 7:271-282.Read more>>
  • Wang et al. MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2. (2017) Cell.Death.Dis. 8:e2792Read more>>
  • Zhang et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. (2016) Cell.Physiol.Biochem. 39:294-302Read more>>
  • Zhang Y et al. Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness. (2018) Biomed Res Int. Nov 1;2018:4025083.Read more>>
  • Wang et al. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment. (2017) J.Ophthalmol. 2017:6067890Read more>>
  • Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2. Read more>>
  • Feng Y et al. Therapeutic Effects of Resveratrol Liposome on Muscle Injury in Rats. Med Sci Monit. 2019 Apr 2;25:2377-2385.Read more>>
  • Ren Y et al. A collagen mimetic peptide-modified hyaluronic acid hydrogel system with enzymatically mediated degradation for mesenchymal stem cell differentiation. Materials Science and Engineering: C,2019, 110276.Read more>>
  • Yu B et al. Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Exp Mol Pathol. 2019 Nov 21;112:104346.Read more>>
  • Chen X et al. Hesperetin derivative attenuates CCl4-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Int Immunopharmacol. 2019 Aug 29;76:105838. Read more>>
  • Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.Read more>>
  • Wu C et al. Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro. Life Sci. 2019 Oct 31;239:116881. Read more>>
  • Wang W et al. LncRNA GAS5 exacerbates renal tubular epithelial fibrosis by acting as a competing endogenous RNA of miR-96-5p. Biomed Pharmacother. 2020 Jan;121:109411. Read more>>
  • Zhan T et al. IL‐9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice. Immunology. 2019 Aug 22. Read more>>
  • Liu C et al. Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair. Nanomedicine (Lond). 2019 Dec 2.Read more>>
  • Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
  • Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
  • Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927. Read more>>
  • Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. Read more>>
  • Xu?C et al. Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms. J Am Soc Nephrol. 2020 May;31(5):946-961.Read more>>
  • Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
  • Yihuan Chenet al. Identification of the collagen family as prognostic biomarkers and immune-associated targets in gastric cancer. Int Immunopharmacol. 2020 Oct;87:106798.Read more>>
  • Mengmeng Liang. et al. Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Mol Ther-Nucl Acids. 2021 Mar;23:1191Read more>>
  • Jinxiu Hu. et al. Role of SIK1 in the transition of acute kidney injury into chronic kidney disease. J Transl Med. 2021 Dec;19(1):1-18Read more>>
  • Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
  • Laiqing Xie. et al. The HIF\1/p53/miRNA\34a/Klotho axis in retinal pigment epithelial cells promotes subretinal fibrosis and exacerbates choroidal neovascularization. J Cell Mol Med. 2021 Feb;25(3):1700-1711Read more>>
  • Zhiqin Deng. et al. High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process. 2021 Jan 15Read more>>
  • Xinlu Zhang. et al. Identification of Transcriptional Variation in Aortic Remodeling Using a Murine Transverse Aortic Constriction (TAC) Model.. Front Cardiovasc Med. 2020; 7: 581362Read more>>
  • Chen Guanyin. et al. Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits. Stem Cells Int. 2020;2020:8822609Read more>>
  • Xiaolan Ouyang. et al. Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions. Stem Cells Dev. 2020 Oct;29(20):1346-1362Read more>>
  • Fei Yin. et al. Effect of Human Umbilical Cord Mesenchymal Stem Cells Transfected with HGF on TGF-1/Smad Signaling Pathway in Carbon Tetrachloride-Induced Liver Fibrosis Rats. Stem Cells Dev. 2020 Oct;29(21):1395-1406Read more>>
  • Kai Zhao. et al. Rhizoma drynariae total flavonoids inhibit the inflammatory response and matrix degeneration via MAPK pathway in a rat degenerative cervical intervertebral disc model. Biomed Pharmacother. 2021 Jun;138:111466Read more>>
  • Deng Zhiqin. et al. Interleukin 1 betaCinduced chloride currents are important in osteoarthritis onset: an in vitro study. Acta Bioch Bioph Sin. 2021 MaRead more>>
  • Zhenjie Huang. et al. Expression of histone deacetylase 2 in tracheal stenosis models and its relationship with tracheal granulation tissue proliferation. Exp Ther Med. 2021 May;21(5):1-1Read more>>
  • Xiao-hong Sun. et al. USP9X deubiquitinates connexin43 to prevent high glucose-induced epithelial-to-mesenchymal transition in NRK-52E cells. Biochem Pharmacol. 2021 Jun;188:114562Read more>>
VALIDATION IMAGES

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. MCF-7 cells probed with Rabbit Anti-Collagen IﾠPolyclonal antibody (bs-10423R)at 1:1000.


This image was kindly submitted by Kandy Velazquez of USC School of Medicine. Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.


Lane 1: Mouse Ovary; Lane 2: NIH/3T3; Lane 3: Rat Liver; Lane 4: 293T; Lane 5: A431; Lane 6: RAW264.7; Probed with Collagen I Polyclonal Antibody (bs-10423R) at 1:300 dilution overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: HepG2 cell lysates probed with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Mouse Placenta lysates; Lane 2: Human U-2OS cell lysates probed with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


U-118 MG (H) cells were treated with or without BFA, 25 μg total protein per lane of cell lysates (see on figure) probed with Collagen I polyclonal antibody, unconjugated (bs-10423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with Collagen I polyclonal antibody, unconjugated (bs-10423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.