bs-10423R
[Primary Antibody]
Collagen 1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Collagen 1
Immunogen Range: 1051-1150/1464
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1277
Swiss Prot: P02452
Source: KLH conjugated synthetic peptide derived from human Collagen I
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 130
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Others
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. Read more>>
- Hu, Jianguo, Biao Zeng, and Xingwei Jiang. "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious."International journal of clinical and experimental pathology 8.2 (2015): 1525.Read more>>
- Zhou, Nan, et al. "Imperatorin derivative OW1 inhibits the upregulation of TGF-β and MMP-2 in renovascular hypertension-induced cardiac remodeling." Experimental and therapeutic medicine 11.5 (2016): 1748-1754.Read more>>
- Zhu, Yi, et al. "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." Medical Science Monitor 22 (2016): 3438-3445.Read more>>
- Jiang, Ke, et al. "Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes." Molecular medicine reports 13.4 (2016): 3567-3573.Read more>>
- Li, Yin, Lin Xiong, and Jianping Gong. "Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells." American Journal of Translational Research 9.6 (2017): 2865-2877.Read more>>
- Miyauchi, Yuya, et al. "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." Scientific Reports 7 (2017).Read more>>
- Belair, David G., et al. "Engineering human cell spheroids to model embryonic tissue fusion in vitro." PloS one 12.9 (2017): e0184155.Read more>>
- Velázquez, Kandy T., et al. "miR155 deficiency aggravates high‐fat diet‐induced adipose tissue fibrosis in male mice." Physiological Reports 5.18 (2017): e13412.Read more>>
- Chiang et al. R1R2 peptide ameliorates pulmonary fibrosis in mice through fibrocyte migration and differentiation. (2017) PLoS.On. 12:e0185811Read more>>
- Liang et al. A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix. (2016) Sci.Re. 6:18205Read more>>
- Lu et al. A novel silk fibroin nanofibrous membrane for guided bone regeneration: a study in rat calvarial defects. (2016) Am.J.Transl.Res. 7:2244-53Read more>>
- Li et al. Morphological effects of MMPs inhibitors on the dentin bonding. (2015) Int.J.Clin.Exp.Med. 8:10793-803Read more>>
- Du et al. MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a. (2015) Sci.Rep. 5:16163Read more>>
- Pan et al. DNA Methylation of PTGIS Enhances Hepatic Stellate Cells Activation and Liver Fibrogenesis. (2018) Front.Pharmacol. 9:553Read more>>
- Li,et al.Effects of sub-chronic exposure to atmospheric PM2.5 on fibrosis, inflammation, endoplasmic reticulum stress and apoptosis in the livers of rats.(2018) Toxicology Research. 7:271-282.Read more>>
- Wang et al. MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2. (2017) Cell.Death.Dis. 8:e2792Read more>>
- Zhang et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. (2016) Cell.Physiol.Biochem. 39:294-302Read more>>
- Zhang Y et al. Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness. (2018) Biomed Res Int. Nov 1;2018:4025083.Read more>>
- Wang et al. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment. (2017) J.Ophthalmol. 2017:6067890Read more>>
- Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2. Read more>>
- Feng Y et al. Therapeutic Effects of Resveratrol Liposome on Muscle Injury in Rats. Med Sci Monit. 2019 Apr 2;25:2377-2385.Read more>>
- Ren Y et al. A collagen mimetic peptide-modified hyaluronic acid hydrogel system with enzymatically mediated degradation for mesenchymal stem cell differentiation. Materials Science and Engineering: C,2019, 110276.Read more>>
- Yu B et al. Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Exp Mol Pathol. 2019 Nov 21;112:104346.Read more>>
- Chen X et al. Hesperetin derivative attenuates CCl4-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Int Immunopharmacol. 2019 Aug 29;76:105838. Read more>>
- Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.Read more>>
- Wu C et al. Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro. Life Sci. 2019 Oct 31;239:116881. Read more>>
- Wang W et al. LncRNA GAS5 exacerbates renal tubular epithelial fibrosis by acting as a competing endogenous RNA of miR-96-5p. Biomed Pharmacother. 2020 Jan;121:109411. Read more>>
- Zhan T et al. IL‐9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice. Immunology. 2019 Aug 22. Read more>>
- Liu C et al. Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair. Nanomedicine (Lond). 2019 Dec 2.Read more>>
- Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
- Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
- Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927. Read more>>
- Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. Read more>>
- Xu?C et al. Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms. J Am Soc Nephrol. 2020 May;31(5):946-961.Read more>>
- Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
- Yihuan Chenet al. Identification of the collagen family as prognostic biomarkers and immune-associated targets in gastric cancer. Int Immunopharmacol. 2020 Oct;87:106798.Read more>>
- Mengmeng Liang. et al. Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Mol Ther-Nucl Acids. 2021 Mar;23:1191Read more>>
- Jinxiu Hu. et al. Role of SIK1 in the transition of acute kidney injury into chronic kidney disease. J Transl Med. 2021 Dec;19(1):1-18Read more>>
- Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
- Laiqing Xie. et al. The HIF\1/p53/miRNA\34a/Klotho axis in retinal pigment epithelial cells promotes subretinal fibrosis and exacerbates choroidal neovascularization. J Cell Mol Med. 2021 Feb;25(3):1700-1711Read more>>
- Zhiqin Deng. et al. High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process. 2021 Jan 15Read more>>
- Xinlu Zhang. et al. Identification of Transcriptional Variation in Aortic Remodeling Using a Murine Transverse Aortic Constriction (TAC) Model.. Front Cardiovasc Med. 2020; 7: 581362Read more>>
- Chen Guanyin. et al. Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits. Stem Cells Int. 2020;2020:8822609Read more>>
- Xiaolan Ouyang. et al. Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions. Stem Cells Dev. 2020 Oct;29(20):1346-1362Read more>>
- Fei Yin. et al. Effect of Human Umbilical Cord Mesenchymal Stem Cells Transfected with HGF on TGF-1/Smad Signaling Pathway in Carbon Tetrachloride-Induced Liver Fibrosis Rats. Stem Cells Dev. 2020 Oct;29(21):1395-1406Read more>>
- Kai Zhao. et al. Rhizoma drynariae total flavonoids inhibit the inflammatory response and matrix degeneration via MAPK pathway in a rat degenerative cervical intervertebral disc model. Biomed Pharmacother. 2021 Jun;138:111466Read more>>
- Deng Zhiqin. et al. Interleukin 1 betaCinduced chloride currents are important in osteoarthritis onset: an in vitro study. Acta Bioch Bioph Sin. 2021 MaRead more>>
- Zhenjie Huang. et al. Expression of histone deacetylase 2 in tracheal stenosis models and its relationship with tracheal granulation tissue proliferation. Exp Ther Med. 2021 May;21(5):1-1Read more>>
- Xiao-hong Sun. et al. USP9X deubiquitinates connexin43 to prevent high glucose-induced epithelial-to-mesenchymal transition in NRK-52E cells. Biochem Pharmacol. 2021 Jun;188:114562Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. Rat aorta, brain, spleen, pancreas, muscle, bladder, lungs, liver, and kidney lysates probed with Rabbit Anti-Collagen IﾠPolyclonal antibody (bs-10423R)at 1:1000.
Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. MCF-7 cells probed with Rabbit Anti-Collagen IﾠPolyclonal antibody (bs-10423R)at 1:1000.
This image was kindly submitted by Kandy Velazquez of USC School of Medicine. Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
Lane 1: Mouse Ovary; Lane 2: NIH/3T3; Lane 3: Rat Liver; Lane 4: 293T; Lane 5: A431; Lane 6: RAW264.7; Probed with Collagen I Polyclonal Antibody (bs-10423R) at 1:300 dilution overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.
Lane 1: HepG2 cell lysates probed with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta lysates; Lane 2: Human U-2OS cell lysates probed with Collagen 1 Polyclonal Antibody, Unconjugated (bs-10423R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Collagen I antibody (bs-10423R) Dilution: 1ug/Test; Protocol Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Collagen I Polyclonal Antibody(bs-10423R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).