Host: Rabbit
Target Protein: NSE
Immunogen Range: 1-100/434
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2026
Swiss Prot: P09104
Source: KLH conjugated synthetic peptide derived from human NSE
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Huiping Wei. et al. Induction of mesenchymal stem cell?like transformation in rat primary glial cells using hypoxia, mild hypothermia and growth factors. Mol Med Rep. 2021 Feb;23(2):1-1Read more>>
- Shan Huang. et al. Effects of Ocular Hypertension on Cytoskeleton and Stiffness of Trabecular Meshwork Cells in Rats. APPL SCI-BASEL. 2022 Jan;12(15):7862Read more>>
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-10445R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-10445R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-10445R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-10445R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Polyclonal Antibody, Unconjugated (bs-10445R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.