bs-10451R [Primary Antibody]
phospho-HSP70 (Tyr41) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-HSP70 (Tyr41)

Modification Site: Tyr41

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 33033304

Swiss Prot: P0DMV8

Source: KLH conjugated synthetic phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr41

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 70


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse epidermis lysates probed with HSP70 (Tyr41) Polyclonal Antibody, unconjugated (bs-10451R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-HSP70 (Tyr41) Antibody(bs-10451R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).