bs-1047R [Primary Antibody]
MyD88 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MyD88

Immunogen Range: 201-296/296


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 17874

Swiss Prot: P22366

Source: KLH conjugated synthetic peptide derived from mouse MyD88

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Isoform 2 is defective in its ability to induce IRAK phosphorylation and NF-kappa-B activation and can function as a negative regulator of activation by IL-1 or lipopolysaccharide (LPS). Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han C et al. Modulation of TLR2 and TLR4 in macrophages following Trichinella spiralis infection.Helminthologia, 55(3), 195–203.Read more>>
  • Wang, Dunjing, et al. "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-κB Activation in Microglial Cells." Inflammation: 1-8.Read more>>
  • Fu, Juanli, et al. "Tetrachlorobenzoquinone exerts neurological pro-inflammatory activity by promoting HMGB1 release, which induces TLR4 clustering within the lipid raft." Toxicological Sciences (2016): kfw124.Read more>>
  • Fu, Juanli, et al. "The acute exposure of tetrachloro-p-benzoquinone (aka chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning." Toxicology (2017).Read more>>
  • Song, Fang, et al. "Correlation of TLR4/MyD88 signaling with early miscarriage." International Journal of Clinical and Experimental Pathology 10.3 (2017): 3601-3608.Read more>>
  • Chen, Gangling, et al. "Limb Remote Ischemic Postconditioning Reduces Ischemia-Reperfusion Injury by Inhibiting NADPH Oxidase Activation and MyD88-TRAF6-P38MAP-Kinase Pathway of Neutrophils." International Journal of Molecular Sciences 17.12 (2016): 1971.Read more>>
  • Li, Chengcheng, et al. "Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway." Scientific Reports 7 (2017).Read more>>
  • Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
  • Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63.Read more>>
  • Wang X et al. Pretreatment with Salvia miltiorrhiza Polysaccharides Protects from Lipopolysaccharides/d-Galactosamine-Induced Liver Injury in Mice through Inhibiting TLR4/MyD88 Signaling Pathway. J Interferon Cytokine Res. 2019 May 10.Read more>>
  • Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.Read more>>
  • Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.Read more>>
  • "Hu NH et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. Journal of Ethnopharmacology.2019."Read more>>
  • Han C et al. Modulation of TLR2 and TLR4 in macrophages following Trichinella spiralis infection.Helminthologia, 55(3), 195–203.Read more>>
  • Liu S et al. Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells. Int J Biol Macromol. 2020 Oct 15;161:797-809.Read more>>
  • Xu D et al. Saturated fatty acids activate the inflammatory signalling pathway in Schwann cells: Implication in sciatic nerve injury. Scand J Immunol. 2020 Aug;92(2):e12896.Read more>>
  • Houpan Song et al. Traditional Chinese Medicine Li-Zhong-Tang accelerates the healing of indomethacin-induced gastric ulcers in rats by affecting TLR-2/MyD88 signaling pathway. J Ethnopharmacol. 2020 Sep 15;259:112979.Read more>>
  • Li Li. et al. Acupuncture at Back-Shu and Front-Mu Acupoints Prevents Gastric Ulcer by Regulating the TLR4/MyD88/NF-B Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8214052Read more>>
  • Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441Read more>>
  • Shun Huang. et al. Design, Synthesis, and Activity Study of Cinnamic Acid Derivatives as Potent Antineuroinflammatory Agents. Acs Chem Neurosci. 2021;12(3):419C429Read more>>
  • Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-B and TGF-/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275Read more>>
  • Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:12405Read more>>
  • Xuemei Zhang. et al. Resveratrol affects the expression of uric acid transporter by improving inflammation. Mol Med Rep. 2021 Aug;24(2):1-9Read more>>
  • Jiali Yang. et al. Baicalin Rescues Cognitive Dysfunction, Mitigates Neurodegeneration, and Exerts Anti-Epileptic Effects Through Activating TLR4/MYD88/Caspase-3 Pathway in Rats. Drug Des Dev Ther. 2021 Jul;15:3163-318Read more>>
  • Rossetto Isabela Maria Urra. et al. Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum. Toxicol Res-Uk. 2021 Aug;10(4):835-849Read more>>
  • Lili Li. et al. Protein overexpression of toll?like receptor 4 and myeloid differentiation factor 88 in oral squamous cell carcinoma and clinical significance. Oncol Lett. 2021 Nov;22(5):1-11Read more>>
  • Xueqing Hu. et al. Tacrolimus alleviates LPS-induced AKI by inhibiting TLR4/MyD88/NF-B signalling in mice. 2021 Dec 09Read more>>
  • Tengteng Yuan. et al. PF-PLC micelles ameliorate cholestatic liver injury via regulating TLR4/MyD88/NF-_B and PXR/CAR/UGT1A1 signaling pathways in EE-induced rats. Int J Pharmaceut. 2022 Mar;615:12148Read more>>
  • Yun-Jiao Shi. et al. Impact of the Plastein Reaction of Casein Hydrolysates in the Presence of Exogenous Amino Acids on Their Anti-Inflammatory Effect in the Lipopolysaccharide-Stimulated Macrophages. Foods. 2022 Jan;11(2):196Read more>>
  • Yongfeng Zhang. et al. Protection against ulcerative colitis and colorectal cancer by evodiamine via anti_inflammatory effects. Mol Med Rep. 2022 May;25(5):1-14Read more>>
  • Xue-Yan Dai. et al. Role of Toll-like Receptor/MyD88 Signaling in Lycopene Alleviated Di-2-ethylhexyl Phthalate (DEHP)-Induced Inflammatory Response. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Canan Ero?lu Gne?. et al. Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells. GENE. 2022 Aug;:146805Read more>>
  • Guirong Chen. et al. The interaction of MD-2 with small molecules in huanglian jiedu decoction play a critical role in the treatment of sepsis.. FRONT PHARMACOL. 2022 Sep;13:947095-947095Read more>>
  • Ming Dong Si. et al. Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-B. PHARM BIOL. 2022 Oct 07Read more>>
  • Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
  • Jian-Zheng Yang. et al. Obeticholic acid protects against methamphetamine-induced anxiety-like behavior by ameliorating microbiota-mediated intestinal barrier impairment. TOXICOLOGY. 2023 Mar;486:153447Read more>>
  • Yi Yan. et al. Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis. Advanced Science. 2023 Feb;:220749Read more>>
  • Xiaoyin Zhang. et al. Proinflammatory S100A9 stimulates TLR4/NF-B signaling pathways causing enhanced phagocytic capacity of microglial cells. IMMUNOL LETT. 2023 Mar;255:54Read more>>
  • Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Ting Li. et al. The Effect of Bergenin on Isonicotinic Acid Hydrazide and Rifampicin-Induced Liver Injury Revealed by RNA Sequencing. MOLECULES. 2023 Jan;28(14):5496Read more>>
  • Yiheng Quan. et al. Bergenin ameliorates diabetic nephropathy in C57BL/6?J mice by TLR4/MyD88/NF-B signalling pathway regulation. TOXICOL APPL PHARM. 2023 Jul;:116633Read more>>
  • Xin-Yue Yang. et al. Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice. J ETHNOPHARMACOL. 2024 Jan;318:117078Read more>>
  • Zhao Hongdi. et al. Electroacupuncture Inhibits Neuroinflammation Induced by Astrocytic Necroptosis Through RIP1/MLKL/TLR4 Pathway in a Mouse Model of Spinal Cord Injury. MOL NEUROBIOL. 2023 Nov;:1-14Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Molt-4 lysates probed with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lung lysates probed with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates probed with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.