bs-10480R [Primary Antibody]
CD79A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD79A

Immunogen Range: 101-226/226


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 973

Swiss Prot: P11912

Source: KLH conjugated synthetic peptide derived from human CD79A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 22


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Sheep
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse spleen lysates probed with Rabbit Anti-CD79A Polyclonal Antibody, Unconjugated (bs-10480R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.


Lane 1: Mouse Lymph node tissue lysates; Lane 2: Rat Lymph node tissue lysates probed with CD79A Polyclonal Antibody, Unconjugated (bs-10480R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.