bs-1049R
[Primary Antibody]
CHRNA7 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CHRNA7
Immunogen Range: 441-502/502
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1139
Swiss Prot: P36544
Source: KLH conjugated synthetic peptide derived from human CHRNA7
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao Y et al. Effect of splenectomy on attenuation of LPS-induced AKI through GTS-21-induced cholinergic anti-inflammatory pathway. Am J Transl Res. 2019 Apr 15;11(4):2540-2549. Read more>>
- Sallam MY et al. Brainstem cholinergic pathways diminish cardiovascular and neuroinflammatory actions of endotoxemia in rats: Role of NFκB/α7/α4β2AChRs signaling. Neuropharmacology. 2019 Jun 25;157:107683. Read more>>
- T.C. Peixoto. et al. Nicotine exposure during lactation causes disruption of hedonic eating behavior and alters dopaminergic system in adult female rats. Appetite. 2021 May;160:105115Read more>>
- Yang Gao. et al. Mechanisms of Renal-Splenic Axis Involvement in Acute Kidney Injury Mediated by the 7nAChR-NF-B Signaling Pathway. 2020 Nov 03Read more>>
- Han Yang. et al. A Novel Targeted and High\Efficiency Nanosystem for Combinational Therapy for Alzheimer's Disease. Adv Sci. 2020 Oct;7(19):1902906Read more>>
- Marta Pernarella. et al. Effects mediated by the 7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells. Eur J Histochem. 2020 Jun 19; 64(Suppl 2): 3159Read more>>
- Mahmoud E. Youssef. et al. 7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996Read more>>
- Martins I. C. A.. et al. Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the 7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response. Mediat Inflamm. 2021;2021:552694Read more>>
- H.E. Burzynski. et al. Pyridostigmine bromide elicits progressive and chronic impairments in the cholinergic anti-inflammatory pathway in the prefrontal cortex and hippocampus of male rats. NEUROBIOL STRESS. 2022 May;18:100446Read more>>
- Suleyma Oliveira Costa. et al. Maternal consumption of a high-fat diet modulates the inflammatory response in their offspring, mediated by the M1 muscarinic receptor. FRONT IMMUNOL. 2023; 14: 1273556Read more>>
- He Ying. et al. Elucidating the mechanisms of formononetin in modulating atherosclerotic plaque formation in ApoE-/- mice. BMC CARDIOVASC DISOR. 2024 Dec;24(1):1-11Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.
HEK 293T cells were stained with CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:500 in PBS and incubated for two hours at room temperature, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:400 dilution for 90 minutes at 37°C. DAPI (5ug/ml, blue) was used to stain the cell nuclei,
Mouse brain lysates probed with Rabbit Anti-CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse lymph node lysates probed with CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Spleen lysates; Lane 4: Mouse Thymus lysates ; Lane 5: Mouse Lymph node lysates probed with CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Adrenal gland lysates; Lane 4: Mouse Adrenal gland lysates; Lane 5: Mouse Placenta lysates; Lane 6: Mouse Lung lysates; Lane 7: Rat Testis lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human SH-SY5Y cell lysates probed with CHRNA7 Polyclonal Antibody, Unconjugated (bs-1049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SH-SY5Y cell Lysates; Lane 2: Human U-87 MG cell Lysates; Lane 3: Human U251 cell Lysates. Probed with CHRNA7 polyclonal Antibody, unconjugated (bs-1049R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (bs-1049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.