DATASHEET
Host:
Rabbit
Target Protein:
Connexin-40
Immunogen Range:
301-358/358
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
2702
Swiss Prot:
P36382
Source:
KLH conjugated synthetic peptide derived from human Connexin-40
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.Read more>>
- Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40.Read more>>
- Zhangchi Liu. et al. Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats. BIOCHEM BIOPH RES CO. 2022 Dec;636:33Read more>>
- Hu Junyi. et al. Multi-omic profiling of clear cell renal cell carcinoma identifies metabolic reprogramming associated with disease progression. NAT GENET. 2024 Feb;56(3):442-457Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Connexin 40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Kidney Lysates; Lane 2: Rat Kidney Lysates. Probed with Connexin-40 polyclonal Antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.