DATASHEET
Host:
Rabbit
Target Protein:
CD55
Immunogen Range:
301-390/390
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
13136
Swiss Prot:
Q61475
Source:
KLH conjugated synthetic peptide derived from mouse CD55
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.
VALIDATION IMAGES
Mouse bladder lysates probed with CD55 Polyclonal Antibody, Unconjugated (bs10549R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human A549 cell lysates probed with CD55 Polyclonal Antibody, Unconjugated (bs-10549R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.