bs-1057R [Primary Antibody]
Integrin Alpha 3 + Beta 1 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Integrin Alpha 3 + Beta 1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3675, 3688

Swiss Prot: P26006, P05556

Source: Mixed KLH conjugated synthetic peptide derived from human Integrin Alpha 3(976-1025/1051) + Beta 1(35-47/798)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

alpha3beta1 integrin is a laminin receptor with apparently diverse functions. In epithelial cells it acts as a receptor for the basement membrane, whereas in neuronal and possibly tumor cells it mediates migration. Interactions of alpha3beta1 integrin with tetraspanin proteins may provide clues to how it transduces signals that affect cell behavior.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 115


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, G., et al. "PAK1 regulates RUFY3-mediated gastric cancer cell migration and invasion." Cell Death & Disease 6.3 (2015): e1682.Read more>>
  • Zhang C et al. CXCR4-Overexpressing Umbilical Cord Mesenchymal Stem Cells Enhance Protection against Radiation-Induced Lung Injury. Stem Cells Int. 2019 Feb 5;2019:2457082. Read more>>
  • Li R et al. Metabolomic analysis reveals metabolic alterations of human peripheral blood lymphocytes by perfluorooctanoic acid. Chemosphere. 2019 Sep 7;239:124810. Read more>>
  • Derya Karabulut. et al. A different perspective on the filtration barrier after kidney stone formation: An immunohistochemical and biochemical study. 2020 Nov 05Read more>>
  • Anqi Liu. et al. Core fucosylation involvement in the paracrine regulation of proteinuria-induced renal interstitial fibrosis evaluated with the use of a microfluidic chip. Acta Biomater. 2022 FebRead more>>
  • Xu, Jun. et al. Mutation of Beclin1 acetylation site at K414 alleviates high glucose-induced podocyte impairment in the early stage of diabetic nephropathy by inhibiting hyperactivated autophagy. Mol Biol Rep. 2022 Feb;:1-8Read more>>
VALIDATION IMAGES

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody (bs-1057R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody (bs-1057R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (bs-1057R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse lung lysates probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (bs-1057R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.


A431 cells were  incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin  Alpha 3 + Beta 1 Antibody(bs-1057R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).