bs-10581R [Primary Antibody]
MMP13 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MMP13

Immunogen Range: 251-350/471


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4322

Swiss Prot: P45452

Source: KLH conjugated synthetic peptide derived from human MMP13

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gong X et al. Synergistically regulated spontaneous calcium signaling is attributed to cartilaginous extracellular matrix metabolism. (2018) J. Cell. Physiol. Oct 28.Read more>>
  • Jin S et al. Sequential Epiphyseal Cartilage Changes of Femoral Heads in C57BL/6 Female Mice Treated with Excessive Glucocorticoids. Cartilage.2020 Dec 3;1947603520978574.Read more>>
  • Ting-ting Yu. et al. Deletion at an 1q24 locus reveals a critical role of long noncoding RNA DNM3OS in skeletal development. Cell Biosci. 2021 Dec;11(1):1-15Read more>>
  • Guoqing Li. et al. Teriparatide ameliorates articular cartilage degradation and aberrant subchondral bone remodeling in DMM mice. J ORTHOP TRANSL. 2023 Jan;38:241Read more>>
  • Tong, Fei. et al. MiR-466d Targeting MMP13 Promotes the Differentiation of Osteoblasts Exposed to a Static Magnetic Field. BIOTECHNOL BIOPROC E. 2023 Feb;:1-1Read more>>
  • Jiang Pinpin. et al. TMT quantitative proteomics reveals key proteins relevant to microRNA-1-mediated regulation in osteoarthritis. PROTEOME SCI. 2023 Dec;21(1):1-11Read more>>
  • Nan Wang. et al. The Sanbi Decoction alleviates intervertebral disc degeneration in rats through intestinal flora and serum metabolic homeostasis modulation. PHYTOMEDICINE. 2024 May;127:15548Read more>>
VALIDATION IMAGES

Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human A431 cell lysates probed with MMP13 Polyclonal Antibody, Unconjugated (bs-10581R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.