bs-10582R-A488 [Conjugated Primary Antibody]
His Tag Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: His Tag

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from His Tag

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 490nm/525nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Tags
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Diana Morais. et al. Liposomal Delivery of Newly Identified Prophage Lysins in a Pseudomonas aeruginosa Model. INT J MOL SCI. 2022 Jan;23(17):10143Read more>>
  • Tiago Gon?alves. et al. Antimicrobial activity of prophage endolysins against critical Enterobacteriaceae antibiotic-resistant bacteria. INT J PHARMACEUT. 2023 Dec;:123758Read more>>
VALIDATION IMAGES