bs-10589R [Primary Antibody]
COL2A1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Collagen alpha-1(II) chain

Immunogen Range: 270-310/1487

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1280

Swiss Prot: P02458

Source: KLH conjugated synthetic peptide derived from human Collagen alpha-1(II) chain

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shi, Yang, et al. "Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes." Integrative Biology 7.3 (2015): 289-297.Read more>>
  • Ren et al. Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo. (2013) Int.J.Mol.Me. 32:1063-8Read more>>
  • Xu et al. Treatment with SiMiaoFang, an anti-arthritis chinese herbal formula, inhibits cartilage matrix degradation in osteoarthritis rat model. (2013) Rejuvenation.Re. 16:364-76Read more>>
  • Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56Read more>>
  • Seidl CI et al. CRISPR-Cas9 targeting of MMP13 in human chondrocytes leads to significantly reduced levels of the metalloproteinase and enhanced type II collagen accumulation.Osteoarthr. Cartil.S1063-4584(18)31458-4. Read more>>
  • Ren Y et al. A collagen mimetic peptide-modified hyaluronic acid hydrogel system with enzymatically mediated degradation for mesenchymal stem cell differentiation. Materials Science and Engineering: C,2019, 110276.Read more>>
  • Ren Y et al. A collagen mimetic peptide-modified hyaluronic acid hydrogel system with enzymatically mediated degradation for mesenchymal stem cell differentiation. Materials Science and Engineering: C,2019, 110276.Read more>>
  • Liu C et al. Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair. Nanomedicine (Lond). 2019 Dec 2.Read more>>
  • Luo P et al. IL‐37b alleviates inflammation in the temporomandibular joint cartilage via IL‐1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. Read more>>
VALIDATION IMAGES

Lane 1: 293T lysates; Lane 2: A549 lysates; Lane 3: LOVO lysates; Lane 4: MCF-7 lysates;Lane 5: HepG2 lysates probed with COL2A1 Polyclonal Antibody, Unconjugated (bs-10589R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Embryo lysates; Lane 2: MG63 lysates probed with COL2A1 Polyclonal Antibody, Unconjugated (bs-10589R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.