bs-10608R

DATASHEET

Host: Rabbit

Target Protein: phospho-MCM2 (Ser53)

Modification Site: Ser53

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4171

Swiss Prot: P49736

Source: KLH conjugated Synthesised phosphopeptide derived from human MCM2 around the phosphorylation site of Ser53

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 101

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Sheep
Pig
Horse
Chicken
Rabbit
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.