bs-1060R [Primary Antibody]
Cytokeratin 5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cytokeratin 5

Immunogen Range: 331-430/590


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3852

Source: KLH conjugated synthetic peptide derived from human CK5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 64


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu X et al. Transdermal Cubic Phases of Metformin Hydrochloride: In Silico and In Vitro Studies of Delivery Mechanisms.Mol Pharm. 2018 Aug 6;15(8):3121-3132.Read more>>
  • Ziwei Luo. et al. miR\203a\3p promotes loureirin A\induced hair follicle stem cells differentiation by targeting Smad1. Anat Rec. 2021 Mar;304(3):531-54Read more>>
  • oyu Chen. et al. Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking. Exp Eye Res. 2021 Jul;:108695Read more>>
  • Jie Kong. et al. Safflower oil body nanoparticles deliver hFGF10 to hair follicles and reduce microinflammation to accelerate hair regeneration in androgenetic alopecia. Int J Pharmaceut. 2022 Mar;616:121537Read more>>
  • Zhengrong Yin. et al. Dissolvable and layered microneedles composed of hyaluronate/rbFGF/CPC effectively improve the treatment effect on recurrent aphthous ulcers. New J Chem. 2022 MaRead more>>
  • Zhang, Xuefei. et al. A multifunctional cholesterol-free liposomal platform based on protopanaxadiol for alopecia therapy. NANO RES. 2022 Jul;:1-13Read more>>
  • Xinxin Lan. et al. Xanthan gum/oil body-microgel emulsions with enhanced transdermal absorption for accelerating wound healing. INT J BIOL MACROMOL. 2022 SepRead more>>
  • Zhou Zhu. et al. Blue-ringed octopus-inspired microneedle patch for robust tissue surface adhesion and active injection drug delivery. SCI ADV. 2023 Jun;9(25)Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti Cytokeratin 5/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat tongue labeled with Rabbit Anti Cytokeratin 5/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 rat heart lysates L2 rat heart lysates probed with Anti Cytokeratin 5/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 64kD. Observed band size:64kD.\n


Lane 1: Mouse Skin lysates; Lane 2: Mouse Uterus lysates; Lane 3: Mouse Urinary bladder lysates; Lane 4: Mouse Esophagus lysates; Lane 5: Rat Skin lysates; Lane 6: Rat Uterus lysates; Lane 7: Rat Urinary bladder lysates; Lane 8: Rat Esophagus probed with Cytokeratin 5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.