bs-1063R [Primary Antibody]
ADRB3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADRB3

Immunogen Range: 310-408/408


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11556

Swiss Prot: P25962

Source: KLH conjugated synthetic peptide derived from mouse ADRB3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.Read more>>
  • Yamashita K et al. Adrenal cortex hypoxia modulates aldosterone production in heart failure. Biochem Biophys Res Commun. 2020 Jan 23. Read more>>
  • Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.Read more>>
  • Clarissa Germano Barp et al. Perivascular adipose tissue phenotype and sepsis vascular dysfunction: Differential contribution of NO, ROS and beta 3-adrenergic receptor. Life Sci. 2020 Aug 1;254:117819.Read more>>
  • Pellegrino Lippiello. et al. Role of 3\adrenergic receptor in the modulation of synaptic transmission and plasticity in mouse cerebellar cortex. J Neurosci Res. 2020 Nov;98(11):2263-2274Read more>>
  • Katarina Andelova. et al. Cardiac Cx43 Signaling Is Enhanced and TGF-β1/SMAD2/3 Suppressed in Response to Cold Acclimation and Modulated by Thyroid Status in Hairless SHRM. BIOMEDICINES. 2022 Jul;10(7):1707Read more>>
  • Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ADRB3/beta 3 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-1063R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-beta 3 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-1063R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.