Host: Rabbit
Target Protein: Troponin T
Immunogen Range: 201-298/298
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7139
Swiss Prot: P45379
Source: KLH conjugated synthetic peptide derived from human Cardiac Troponin T
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 36
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Zhao et al. Rho-associated protein kinases play an important role in the differentiation of rat adipose-derived stromal cells into cardiomyocytes in vitro. (2014) PLoS.One. 9:e115191Read more>>
- Jia, Li. et al. A Novel Splicing Mutation c.3351 G_>_A in the Cardiac Transcription Factor NKX2-5 Leads to Familial Atrial Septal Defect Through miR-19 and PYK2. Stem Cell Reviews and Reports. 2022 Jul;:1-16Read more>>
- Wenhao Han. et al. Targeting Myocardial Mitochondria-STING-Polyamine Axis Prevents Cardiac Hypertrophy in Chronic Kidney Disease. JACC-BASIC TRANSL SC. 2022 AugRead more>>
- Li Chen. et al. l-Arginine and l-lysine can weaken the intermolecular interactions of main myofibrillar proteins: the roles in improving the tenderness of pork Longissimus lumborum muscle. INT J FOOD SCI TECH. 2023 ApRead more>>
- Dr. Rabia Onbas. et al. Biopatterning of 3D Cellular Model by Contactless Magnetic Manipulation for Cardiotoxicity Screening. Tissue Engineering Part A. 2023 Nov 17Read more>>
Rat H9C2 cells probed with Rabbit Anti-Troponin T Polyclonal Antibody, Unconjugated (bs-10648R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Troponin T Polyclonal Antibody, Unconjugated (bs-10648R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Troponin T Polyclonal Antibody, Unconjugated (bs-10648R) at 1:500 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei
Mouse heart lysates probed with Rabbit Anti-Troponin T Polyclonal Antibody, Unconjugated (bs-10648R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Mouse Heart Lysates; Lane 2: Rat Heart Lysates. Probed with Cardiac Troponin T polyclonal Antibody, unconjugated (bs-10648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Troponin T ) Polyclonal Antibody, Unconjugated (bs-10648R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.