bs-10657R [Primary Antibody]
PI3 Kinase p110 beta Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: PI3 Kinase p110 beta

Immunogen Range: 971-1070/1070


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5291

Swiss Prot: P42338

Source: KLH conjugated synthetic peptide derived from human PI3 Kinase p110 beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 123


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiang S et al. Hesperetin as an adjuvant augments protective antitumor immunity responses in B16F10 melanoma by stimulating cytotoxic CD8+ T cells. Scandinavian Journal of Immunology, 2020: e12867.Read more>>
  • Jiang S et al. Hesperetin as an adjuvant augments protective antitumor immunity responses in B16F10 melanoma by stimulating cytotoxic CD8+ T cells. Scandinavian Journal of Immunology, 2020: e12867.Read more>>
  • Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14Read more>>
  • Le Tian. et al. Luteolin as an adjuvant effectively enhances CTL anti-tumor response in B16F10 mouse model. Int Immunopharmacol. 2021 May;94:107441Read more>>
  • Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
  • Yuling Tong. et al. Zuojin Pill ameliorates chronic atrophic gastritis induced by MNNG through TGF-1/PI3K/Akt axis. J Ethnopharmacol. 2021 May;271:113893Read more>>
  • Wenqian Xie. et al. Seasonal expressions of ER, ER, EGF, EGFR, PI3K and Akt in the scent glands of the muskrats (Ondatra zibethicus). J Steroid Biochem. 2021 Oct;213:105961Read more>>
  • Siyuan Liu. et al. _-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinasemediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production. J Dairy Sci. 2022 FebRead more>>
  • Lingling Dong. et al. Anti-inflammatory effect of Rhein on ulcerative colitis via inhibiting PI3K/Akt/mTOR signaling pathway and regulating gut microbiota. 2022 Mar 01Read more>>
  • Xu Zongying. et al. Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory Cytokines and the VEGF-PI3K/Akt-eNOS Signaling Pathway. EVID-BASED COMPL ALT. 2022;2022:4621131Read more>>
  • Yusong Ge. et al. L-arginine stimulates the proliferation of mouse mammary epithelial cells and the development of mammary gland in pubertal mice by activating the GPRC6A/PI3K/AKT/mTOR signalling pathway. J ANIM PHYSIOL AN N. 2022 May 26Read more>>
  • Chen Feng. et al. Xiao-Chai-Hu Decoction Ameliorates Poly (I:C)-Induced Viral Pneumonia through Inhibiting Inflammatory Response and Modulating Serum Metabolism. EVID-BASED COMPL ALT. 2022;2022:1240242Read more>>
  • Xiaofei Zhou. et al. Chlorogenic Acid Prevents Hyperuricemia Nephropathy via Regulating TMAO-Related Gut Microbes and Inhibiting the PI3K/AKT/mTOR Pathway. J AGR FOOD CHEM. 2022;70(33):1018210193Read more>>
  • Dongfang Lv. et al. Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.. FRONT PHARMACOL. 2022 Sep;13:961011-961011Read more>>
  • Xiao-Jiao Chen. et al. Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways. MOLECULES. 2022 Jan;27(19):6435Read more>>
  • Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringers solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331Read more>>
  • Xiaoyi Meng. et al. Hugan Buzure Induces Autophagy and Apoptosis in Hepatocellular Carcinoma by Inhibiting PI3K/Akt/mTOR Signaling Pathway. EVID-BASED COMPL ALT. 2022 Dec 19;2022:1618491Read more>>
  • Chenglin Li. et al. Ranitidine as an adjuvant regulates macrophage polarization and activates CTLs through the PI3K-Akt2 signaling pathway. INT IMMUNOPHARMACOL. 2023 Mar;116:109729Read more>>
  • Xinyang Fan. et al. CEBPA-Regulated Expression of SOCS1 Suppresses Milk Protein Synthesis through mTOR and JAK2-STAT5 Signaling Pathways in Buffalo Mammary Epithelial Cells. FOODS. 2023 Jan;12(4):708Read more>>
  • Xinyang Fan. et al. MiR-190a regulates milk protein biosynthesis through the mTOR and JAK2CSTAT5 signaling pathways by targeting PTHLH in buffalo mammary epithelial cells. J FUNCT FOODS. 2023 Mar;102:105451Read more>>
  • Tingting Zhao. et al. Yishen Huashi granule modulated lipid metabolism in diabetic nephropathy via PI3K/AKT/mTOR signaling pathways.. HELIYON. 2023 Mar;9(3):e14171-e14171Read more>>
  • Zhao Zhongwei. et al. Anticancer potential of Bacillus coagulans MZY531 on mouse H22 hepatocellular carcinoma cells via anti-proliferation and apoptosis induction. BMC Complementary Medicine and Therapies. 2023 Dec;23(1):1-9Read more>>
  • Jiani Liu. et al. Loke zupa decoction attenuates bronchial EMT-mediated airway remodelling in chronic asthma through the PI3K-Akt/HIF-1 signaling pathway. PHARM BIOL. 2023;61(1):1332-1342Read more>>
  • Huijuan Qu. et al. Components study on gastroprotective effect and holistic mechanism of the herbal pair Alpinia officinarum - Cyperus rotundus based on spectrum-effect relationship and integrated transcriptome and metabolome analyses. J ETHNOPHARMACOL. 2023 Nov;:117494Read more>>
  • Feng Qiu. et al. The mechanism of Chebulae Fructus Immaturus promote diabetic wound healing based on network pharmacology and experimental verification. J ETHNOPHARMACOL. 2024 Mar;322:117579Read more>>
  • Zhenjie Wang. et al. Effect of sodium bicarbonate Ringer's solution on lung injury in rats with traumatic hemorrhagic shock. J BIOCHEM MOL TOXIC. 2023 Dec;:e23608Read more>>
  • Liu Pei-Yu. et al. Total flavonoids of Astragalus protects glomerular filtration barrier in diabetic kidney disease. CHIN MED-UK. 2024 Dec;19(1):1-13Read more>>
  • Qiushi Xu. et al. Role of diacylglycerol O-acyltransferase 1 (DGAT1) in lipolysis and autophagy of adipose tissue from ketotic dairy cows. J DAIRY SCI. 2024 FebRead more>>
  • Fu Yuhang. et al. Effect of umbilical cord blood-mononuclear cells on knee osteoarthritis in rabbits. J ORTHOP SURG RES. 2024 Dec;19(1):1-9Read more>>
  • Zhilan Hu. et al. Effects of asiaticoside on the model of gestational diabetes mellitus in HTR-8/svneo cells via PI3K/AKT pathway. J OBSTET GYNAECOL. 2024 24Read more>>
  • Yanan Gao. et al. Butyrate improves recovery from experimental necrotizing enterocolitis by metabolite hesperetin through potential inhibition the PI3K-Akt pathway. BIOMED PHARMACOTHER. 2024 Jul;176:116876Read more>>
  • Daohao Tang. et al. Integrated Multi-Omics Analysis Reveals Mountain-Cultivated Ginseng Ameliorates Cold-Stimulated Steroid-Resistant Asthma by Regulating Interactions among Microbiota, Genes, and Metabolites. INT J MOL SCI. 2024 Jan;25(16):911Read more>>
  • Zhao JinFeng. et al. Synthesis, biological activities and mechanistic studies of C20-ketone pachysandra alkaloids as anti-hepatocellular carcinoma agents. MOL DIVERS. 2024 Aug;:1-21Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon、; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (bs-10657R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with PI3 kinase p110 beta polyclonal antibody, unconjugated (bs-10657R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.