bs-10666R [Primary Antibody]
phospho-AMPK alpha-1 (Ser184) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-AMPK alpha-1 (Ser184)

Specificity: Based on the high protein similarity this antibody will bind AMPK alpha 1 when phorphorylated at Ser184 and AMPK alpha 2 when phosphrylated at Ser173.

Immunogen Range: 170-200/559


Modification Site: Ser184

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5562

Swiss Prot: Q13131

Source: KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 1 around the phosphorylation site of Ser184

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1ug/Test)
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 64 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiang T et al. Protein-bound Anthocyanin Compounds of Purple Sweet Potato (p-BAC-PSP) Ameliorate Hyperglycemia by Regulating Hepatic Glucose Metabolism in High Fat Diet/Streptozotocin-Induced Diabetic Mice. J Agric Food Chem. 2020 Feb 12;68(6):1596-1608.Read more>>
  • Huan Deng. et al. Low selenium and T-2 toxin may be involved in the pathogenesis of Kashin-Beck disease by affecting AMPK/mTOR/ULK1 pathway mediated autophagy. ECOTOX ENVIRON SAFE. 2024 Jul;279:116503Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AMPK alpha-1(Ser184) Polyclonal Antibody, Unconjugated (bs-10666R) at 1:400 overnight at 4°C, DAB staining.