bs-10699R [Primary Antibody]
CD8a Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD8

Immunogen Range: 101-200/247


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12525

Swiss Prot: P01731

Source: KLH conjugated synthetic peptide derived from human CD8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Song et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine on metastatic breast cancer in a mouse model. (2018) Oncol.Lett. 15:6831-6838Read more>>
  • Song et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine on metastatic breast cancer in a mouse model. (2018) Oncol.Lett. 15:6831-6838Read more>>
  • Pan Y et al. Amino-Modified Polymer Nanoparticles as Adjuvants to Activate the Complement System and to Improve Vaccine Efficacy in Vivo. Biomacromolecules. 2019 Sep 9;20(9):3575-3583.Read more>>
  • Chong Zhang. et al. Histological tumor response to neoadjuvant chemotherapy correlates to Immunoscore in colorectal cancer liver metastases patients. 2021 Aug 18Read more>>
  • Shifa Zhang. et al. Impact of the combination of sintilimab and chemotherapy on the tumor and paratumor PD-L1, IDO, TIM-3, FOXP3+ and CD8 expressions in patients with advanced esophageal squamous cell carcinoma. THORAC CANCER. 2022 OcRead more>>
  • Wangyou Feng. et al. CDK4/6i enhances the antitumor effect of PD1 antibody by promoting TLS formation in ovarian cancer. HELIYON. 2023 Sep;9:e1976Read more>>
  • Lei Wang. et al. STING Agonist-Loaded Nanoparticles Promotes Positive Regulation of Type I Interferon-Dependent Radioimmunotherapy in Rectal Cancer. ADV SCI. 2023 Dec;:2307858Read more>>
VALIDATION IMAGES

Mouse lymph node lysate probed with CD8a Polyclonal Antibody (bs-10699R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Rat Thymus tissue lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-10699R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.