bs-1074R
[Primary Antibody]
Nrf2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Nrf2
Immunogen Range: 401-500/605
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4780
Swiss Prot: Q16236
Source: KLH conjugated synthetic peptide derived from human Nrf2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 68
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li P et al. Selenium Yeast Alleviates Ochratoxin A-Induced Hepatotoxicity via Modulation of the PI3K/AKT and Nrf2/Keap1 Signaling Pathways in Chickens. Toxins (Basel). 2020 Feb 25;12(3).Read more>>
- Bao-Hong, L. et al. "Graptopetalum paraguayense and resveratrol ameliorates 3 carboxymethyllysine (CML)-induced pancreas dysfunction and 4 hyperglycemia." Food and Chemical Toxicology. (2013).Read more>>
- Lee, Bao-Hong, et al. "Suppression of dimerumic acid on hepatic fibrosis caused from carboxymethyl-lysine (CML) by attenuating oxidative stress depends on Nrf2 activation in hepatic stellate cells (HSCs)." Food and Chemical Toxicology (201Read more>>
- Lee, Bao-Hong, et al. "< i> Graptopetalum paraguayense</i> and resveratrol ameliorates carboxymethyllysine (CML)-induced pancreas dysfunction and hyperglycemia." Food and Chemical Toxicology 62 (2013): 492-498.Read more>>
- Lee, Bao-Hong, et al. "Suppression of dimerumic acid on hepatic fibrosis caused from carboxymethyl-lysine (CML) by attenuating oxidative stress depends on Nrf2 activation in hepatic stellate cells (HSCs)." Food and Chemical Toxicology 62 (2013): 413-419.Read more>>
- Hsu, Wei-Hsuan, et al. "Monascin and AITC attenuate methylglyoxal-induced PPARγ phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation." Journal of agricultural and food chemistry (2013).Read more>>
- Lee, Bao-Hong, et al. "Effects of monascin on anti-inflammation mediated by Nrf2 activation in advanced glycation endproducts-treated THP-1 monocytes and methylglyoxal-treated Wistar rats." Journal of Agricultural and Food Chemistry (2013).Read more>>
- Cheng, An-Sheng, Yu-Hsiang Cheng, and Tsu-Liang Chang. "Resveratrol protects RINm5F pancreatic cells from methylglyoxal-induced apoptosis." Journal of Functional Foods (2013).Read more>>
- Wang, Yan, et al. "The Chronic Effects of Low Lead Level on The Expressions of Nrf2 and Mrp1 of The Testes in The Rats." Environmental Toxicology and Pharmacology (2012).Read more>>
- Lee, Chia-Chen, Bao-Hong Lee, and She-Ching Wu. "Actinidia callosa peel (kiwi fruit) ethanol extracts protected neural cells apoptosis induced by methylglyoxal through Nrf2 activation." Pharmaceutical Biology 0 (2014): 1-9.Read more>>
- Cui, Y., et al. "Role of Nuclear Factor Erythroid 2-Like 2 in the Induction of Cytochrome P450 2a5 in Vivo of Nonalcoholic Fatty Liver Disease." Journal of Diabetes & Metabolism 6.488 (2015): 2.Read more>>
- Wang, Peng, et al. "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzymes expression via PI3K/AKT and ERK1/2 pathway." Biochimica et Biophysica Acta (BBA)-General Subjects (2015).Read more>>
- Yu, Haijie, et al. "Triptolide Attenuates Myocardial Ischemia/Reperfusion Injuries in Rats by Inducing the Activation of Nrf2/HO-1 Defense Pathway."Cardiovascular Toxicology (2015): 1-11.Read more>>
- Lv, Runxiao, et al. "Neuroprotective effect of allicin in a rat model of acute spinal cord injury." Life Sciences (2015).Read more>>
- Jiang, Wu, et al. "Protective Effects of Asiatic Acid Against Spinal Cord Injury-Induced Acute Lung Injury in Rats." Inflammation: (2016) 1-9.Read more>>
- Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597.Read more>>
- Zeng, X-P., et al. "Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity." Transplantation Proceedings. Vol. 49. , 2017.Read more>>
- Zhang, Jin-ming, et al. "Nrf2 Is Crucial For The Down-Regulation Of Cyp7a1 Induced By Arachidonic Acid In Hepg2 Cells." Environmental Toxicology and Pharmacology (2017).Read more>>
- Yu, Dan, et al. "In vitro the differences of inflammatory and oxidative reactions due to sulfur mustard induced acute pulmonary injury underlying intraperitoneal injection and intratracheal instillation in rats." International Immunopharmacology 47 (2017): 78-87.Read more>>
- Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.Read more>>
- Zhao, Lei, et al. "Protective effects of Lactobacillus plantarum C88 on chronic ethanol-induced liver injury in mice." Journal of Functional Foods 35 (2017): 97-104.Read more>>
- Tao, Kai, Jinghai Chen, and Lingyun Wang. "Effects of berberine on the expressions of NRF2 and HO-1 in endothelial cells of diabetic rat." Biomedical Research 28.9 (2017): 3860-3864.Read more>>
- Zhang, Bing, et al. "Nrf2 mediates the protective effects of homocysteine by increasing the levels of GSH content in HepG2 cells." Molecular Medicine Reports.Read more>>
- Cao, Zhen, et al. "Cyanidin Suppresses Autophagic Activity Regulating Chondrocyte Hypertrophic Differentiation." Journal of Cellular Physiology(2017).Read more>>
- Xu, Wei, et al. "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson’s Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." Cellular Physiology and Biochemistry 44.6 (2017): 2091-2104.Read more>>
- Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
- Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
- Oyagbemi AA et al. Ameliorative effect of Rutin on sodium fluoride‐induced hypertension through modulation of Kim‐1/NF‐κB/Nrf2 signaling pathway in rats.(2018)Environ Toxicol.Read more>>
- Xu Z et al. Grape seed extracts attenuate retinal Müller cell gliosis in streptozotocin-diabetic rats.(2018) Journal of Functional Foods, 50, 45–52. Read more>>
- Liu and Yan Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway. (2018) Int.J.Mol.Med. 42:2315-2328Read more>>
- Peng XP et al. The protective effect of oleanolic acid on NMDA-induced MLE-12 cells apoptosis and lung injury in mice by activating SIRT1 and reducing NF-κB acetylation.Int Immunopharmacol. 2019 May;70:520-529.Read more>>
- Ola-Davies OE et al. Ameliorative effects of Annona muricata Linn. (Annonaceae) against potassium dichromate-induced hypertension in vivo: involvement of Kim-1/p38 MAPK/Nrf2 signaling. J Basic Clin Physiol Pharmacol. 2019 May 3.Read more>>
- Zhao C et al. Dimethyl itaconate protects against lippolysacchride-induced mastitis in mice by activatingMAPKs and Nrf2 and inhibiting NF-κB signaling pathways. Microb Pathog. 2019 Aug;133:103541.Read more>>
- Filev AD et al. Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress. Oxid Med Cell Longev. 2019 Jul 8;2019:1245749. Read more>>
- Jiang S et al. Effect of Illicium verum or Eucommia ulmoides leaf extracts on the anti-stress ability, and mRNA and protein expression of Nrf2 and TNF-α in Duroc × Landrace × Yorkshire and Chinese native Licha-black nursery piglets. J Anim Physiol Anim Nutr (Berl). 2019 Oct 31. Read more>>
- Li X et al. Endogenous ET-1 promotes ANP secretion through activation of COX2-L–PGDS–PPARγ signaling in hypoxic beating rat atria. Peptides. 2019 Sep 18;122:170150. Read more>>
- Li J et al. Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Vet Res. 2019 Oct 21;50(1):83.Read more>>
- Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
- Jiang Z et al. The Essential Oils and Eucalyptol From Artemisia vulgaris L. Prevent Acetaminophen-Induced Liver Injury by Activating Nrf2-Keap1 and Enhancing APAP Clearance Through Non-Toxic Metabolic Pathway. Front Pharmacol. 2019 Jul 25;10:782. Read more>>
- Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
- Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239.Read more>>
- Hu Y et al. Effects of Essential Trace Elements and Oxidative Stress on Endemic Arsenism Caused by Coal Burning in PR China. Biol Trace Elem Res. 2020 Jan 20.Read more>>
- Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268. Read more>>
- Wang C et al. A novel acidic polysaccharide from the residue of Panax notoginseng and its hepatoprotective effect on alcoholic liver damage in mice. Int J Biol Macromol. 2020 Feb 6;149:1084-1097.Read more>>
- Li YH et al. GanMeijian Ameliorates Lipid Accumulation and Oxidative Damage in Alcoholic Fatty Liver Disease in Wistar Rats. Life Sci. 2020 Apr30;255:117721.Read more>>
- Li P et al. Selenium Yeast Alleviates Ochratoxin A-Induced Hepatotoxicity via Modulation of the PI3K/AKT and Nrf2/Keap1 Signaling Pathways in Chickens. Toxins (Basel). 2020 Feb 25;12(3).Read more>>
- Shmarina et al. NRF2 and HMOX1 Gene Expression against the Background of Systemic Oxidative Stress in Patients with Acute Psychosis. Russian Journal of Genetics, 2020, 56(1), 96–102. Read more>>
- Svetlana V Kostyuket al. Effects of Functionalized Fullerenes on ROS Homeostasis Determine Their Cytoprotective or Cytotoxic Properties. Nanomaterials (Basel). 2020 Jul 19;10(7):1405.Read more>>
- Yanxia Gaoet al. Octreotide alleviates pancreatic damage caused by paraquat in rats by reducing inflammatory responses and oxidative stress. Environ Toxicol Pharmacol. 2020 Nov;80:103456.Read more>>
- Wu Y et al. LncRNAs activate longevity regulation pathway due to aging of Leydig cells caused by DEHP exposure: A transcriptome-based studyEcotoxicol Environ Saf.2021 Feb;209:111798.Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse myocardium labeled with (bs-1074R) Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size:66kD.\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Liver lysates; Lane 3: Rat Liver lysates; Lane 4: Human Siha cell lysates; Lane 5: Human HepG2 cell lysates probed with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hep-G2 (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (bs-1074R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The Hela（treated with MG-132 (10 μM,10h) ） (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Nrf2 antibody (bs-1074R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela（treated with MG-132 (10 μM,10h) ）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Nrf2) polyclonal Antibody, unconjugated (bs-1074R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.