bs-10750R [Primary Antibody]
C1Q Polyclonal Antibody
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Host: Rabbit

Target Protein: C1Q

Immunogen Range: 200-245/245


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 714

Swiss Prot: P02747

Source: KLH conjugated synthetic peptide derived from human C1Q

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 26


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-C1Q Polyclonal Antibody, Unconjugated (bs-10750R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-C1Q Polyclonal Antibody, Unconjugated (bs-10750R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining