DATASHEET
Host:
Rabbit
Target Protein:
1-Oct
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5451
Source:
KLH conjugated synthetic peptide derived from human OCT1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Oct1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR and interacts with NR3C1, AR and PGR. Oct1 is Ubiquitous and belongs to the POU transcription factor family class 2 subfamily. Oct1 contains 1 homeobox domain.
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-OCT1 Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-OCT1 Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (OCT-1 ) Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.