bs-10943R [Primary Antibody]
IL17E Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL17E

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 64806

Swiss Prot: Q9H293

Source: KLH conjugated synthetic peptide derived from human IL17E

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zewei Zhao. et al. IL-25 ameliorates acute cholestatic liver injury via promoting hepatic bile acid secretion. CYTOKINE. 2022 Oct;158:155979Read more>>
  • Min-jie Gong. et al. Melatonin reduces IL-33 and TSLP expression in human nasal epithelial cells by scavenging ROS directly. IMMUN INFLAMM DIS. 2023 Feb;11(2):e788Read more>>
  • Meiping Zhang. et al. Hypoxia induces the production of epithelial-derived cytokines in eosinophilic chronic rhinosinusitis with nasal polyps. INT IMMUNOPHARMACOL. 2023 Aug;121:110559Read more>>
VALIDATION IMAGES

Mouse spleen lysates probed with IL17E Polyclonal Antibody, Unconjugated (bs-10943R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Lung Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Anti-IL17E Polyclonal Antibody, Unconjugated (bs-10943R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.