bs-1095R [Primary Antibody]
Orexin receptor 1+2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Orexin receptor 1+2

Immunogen Range: 321-425/444


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3062

Swiss Prot: O43614

Source: KLH conjugated synthetic peptide derived from human Orexin receptor 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. [provided by RefSeq, Jan 2009]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 50


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hui Fenget al. Orexin signaling modulates synchronized excitation in the sublaterodorsal tegmental nucleus to stabilize REM sleep. Nat Commun. 2020 Jul 21;11(1):3661.Read more>>
  • Qi-Cheng Qiao. et al. Orexin recruits non-selective cationic conductance and endocannabinoid to dynamically modulate firing of caudal pontine reticular nuclear neurones. J PHYSIOL-LONDON. 2023 JulRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD200R/Orexin receptor Polyclonal Antibody, Unconjugated (bs-1095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat kidney labeled with Anti-CD200R/Orexin receptor Polyclonal Antibody, Unconjugated (bs-1095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse cerebral cortex lysates; Lane 2: Mouse hippocampus lysates; Lane 3: Mouse brain lysates; Lane 4: rat Brain lysates probed with Orexin receptor 1+2 Polyclonal Antibody, Unconjugated (bs-1095R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates probed with Orexin receptor 1+2 Polyclonal Antibody, Unconjugated (bs-1095R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.