bs-10966R [Primary Antibody]
Actin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Actin

Immunogen Range: 3-100/377


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 58

Swiss Prot: P68133

Source: KLH conjugated synthetic peptide derived from human ACTA1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sheng Y et al. Biomed Pharmacother. 2020 Jan;121:109610. Read more>>
  • Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. Read more>>
  • Junxia An. et al. A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen?thawed embryo transfer. Exp Ther Med. 2020 Sep;20(3):2191-2199Read more>>
  • Liqin Anet al. Benzo[a]pyrene injures BMP2-induced osteogenic differentiation of mesenchymal stem cells through AhR reducing BMPRII. Ecotoxicol Environ Saf. 2020 Oct 15;203:110930.Read more>>
  • Ruixi Luo. et al. Mesenchymal stem cells alleviate palmitic acid-induced endothelial-to-mesenchymal transition by suppressing endoplasmic reticulum stress. Am J Physiol-Endoc M. 2020 Nov;319(6):E961-E98Read more>>
  • Ramendu Parial. et al. Role of epigenetic m6A RNA methylation in vascular development: mettl3 regulates vascular development through PHLPP2/mTOR\AKT signaling. Faseb J. 2021 May;35(5):e21465Read more>>
  • Kaiqiang Fu. et al. Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway. Res Vet Sci. 2021 May;136:22Read more>>
  • Shaohua Hou. et al. Design, synthesis and biological evaluation of 1H-indazole derivatives as novel ASK1 inhibitors. Eur J Med Chem. 2021 Aug;220:113482Read more>>
  • Haina Gao. et al. 3,5,6-trichloro-2-pyridinol intensifies the effect of chlorpyrifos on the paracrine function of Sertoli cells by preventing binding of testosterone and the androgen receptor. Toxicology. 2021 Aug;460:152883Read more>>
  • Hu, Su-Qin. et al. Wuzi-Yanzong prescription alleviates spermatogenesis disorder induced by heat stress dependent on Akt, NF-B signaling pathway. Sci Rep-Uk. 2021 Sep;11(1):1-15Read more>>
  • Ming Hu. et al. Human Cytomegalovirus Infection Activates Glioma Activating Transcription Factor 5 via microRNA in a Stress-Induced Manner. Acs Chem Neurosci. 2021;12(20):3947C3956Read more>>
  • Shi, Yesi. et al. Biomimetic nanoparticles blocking autophagy for enhanced chemotherapy and metastasis inhibition via reversing focal adhesion disassembly. J Nanobiotechnol. 2021 Dec;19(1):1-17Read more>>
  • Yanxiong Huo. et al. Bifidobacterium animalis subsp. lactis A6 Enhances Fatty Acid β-Oxidation of Adipose Tissue to Ameliorate the Development of Obesity in Mice. Nutrients. 2022 Jan;14(3):598Read more>>
  • Gong, Hui. et al. miR-146a impedes the anti-aging effect of AMPK via NAMPT suppression and NAD+/SIRT inactivation. Signal Transduct Tar. 2022 Mar;7(1):1-13Read more>>
  • Meng Zhang. et al. Moutan Cortex polysaccharide ameliorates diabetic kidney disease via modulating gut microbiota dynamically in rats. Int J Biol Macromol. 2022 May;206:849Read more>>
  • Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453Read more>>
  • Huang Jian. et al. Icariside II Attenuates Methamphetamine-Induced Neurotoxicity and Behavioral Impairments via Activating the Keap1-Nrf2 Pathway. Oxid Med Cell Longev. 2022;2022:8400876Read more>>
  • Antonia Giacco. et al. Mild Endurance Exercise during Fasting Increases Gastrocnemius Muscle and Prefrontal Cortex Thyroid Hormone Levels through Differential BHB and BCAA-Mediated BDNF-mTOR Signaling in Rats. Nutrients. 2022 Jan;14(6):1166Read more>>
  • Shi C. et al. Proteomic Analysis of Plasma-Derived Extracellular Vesicles From Mice With <i>Echinococcus granulosus</i> at Different Infection Stages and Their Immunomodulatory Functions.. Front Cell Infect Mi. 2022 Mar;12:805010-80501Read more>>
  • Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-_B and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199Read more>>
  • Guo-Dong Wu. et al. Cordyceps Improves Obesity and its Related Inflammation via Modulation of Enterococcus cecorum Abundance and Bile Acid Metabolism. 2022 Mar 1Read more>>
  • Weitong Xu. et al. Pan-mTOR inhibitors sensitize the senolytic activity of navitoclax via mTORC2 inhibition-mediated apoptotic signaling. BIOCHEM PHARMACOL. 2022 Jun;200:115045Read more>>
  • Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227Read more>>
  • Ya-Nan Gao. et al. Multi-Omics Reveal Additive Cytotoxicity Effects of Aflatoxin B1 and Aflatoxin M1 toward Intestinal NCM460 Cells. TOXINS. 2022 Jun;14(6):368Read more>>
  • Jianwei Lei. et al. The protective mechanism of protein kinase R to inhibit neuronal ferroptosis in cerebral injury from subarachnoid hemorrhage. BRAIN BEHAV. 2022 Jul;:e2722Read more>>
  • Zhou, Taocheng. et al. Leonurine Alleviates Alcoholic Steatohepatitis Through the TLR4/NF-B Signalling Pathway. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy. 2022 Aug;:1-15Read more>>
  • Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488Read more>>
  • Wang Huan. et al. CTC1 OB-B interaction with TPP1 terminates telomerase and prevents telomere overextension. NUCLEIC ACIDS RES. 2023 ApRead more>>
  • Ying-Cai Zhao. et al. N-3 PUFA Deficiency from Early Life to Adulthood Exacerbated Susceptibility to Reactive Oxygen Species-Induced Testicular Dysfunction in Adult Mice. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Chang Xiao. et al. Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy. ACTA PHARM SIN B. 2023 MayRead more>>
  • Xue-sheng Pan. et al. Kupffer cell pyroptosis mediated by METTL3 contributes to the progression of alcoholic steatohepatitis. FASEB J. 2023 May;37(6):e22965Read more>>
  • Yanhui Jia. et al. The deacetylation of Akt by SIRT1 inhibits inflammation in macrophages and protects against sepsis. EXP BIOL MED. ;()Read more>>
  • Ying-Cai Zhao. et al. Supplementation of n-3 PUFAs in Adulthood Attenuated Susceptibility to Pentylenetetrazol Induced Epilepsy in Mice Fed with n-3 PUFAs Deficient Diet in Early Life. MAR DRUGS. 2023 Jun;21(6):354Read more>>
  • Ming Li. et al. Fibroblast activating protein promotes the proliferation, migration, and activation of fibroblasts in oral submucous fibrosis. ORAL DIS. 2023 JuRead more>>
  • Jiawen Li. et al. Analysis of the mRNA export protein ZC3H11A in HCMV infection and pan-cancer. FRONT MICROBIOL. 2023; 14: 1296725Read more>>
  • Chuanting Xu. et al. Histone deacetylase-mediated silencing of PSTPIP2 expression contributes to AAI-induced PANoptosis. BRIT J PHARMACOL. 2023 DecRead more>>
  • Cheng-Ling Xu. et al. Antibiotic administration aggravates asthma by disrupting gut microbiota and the intestinal mucosal barrier in an asthma mouse model. EXP THER MED. 2024 Apr;27(4):1-15Read more>>
VALIDATION IMAGES

Mouse heart lysates probed with Actin/ACTA1 Polyclonal Antibody, Unconjugated (bs-10966R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.