Host: Rabbit
Target Protein: SKP2
Immunogen Range: 351-424/424
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6502
Swiss Prot: Q13309
Source: KLH conjugated synthetic peptide derived from human Skp2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
- Yang, Wei‐Rong, et al. "mTOR is involved in 17β‐estradiol‐induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." Molecular Reproduction and Development (2015).Read more>>
- Yang, Wei‐Rong, et al. "mTOR is involved in 17β‐estradiol‐induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." Molecular Reproduction and Development (2015).Read more>>
- Jiao, Zhang Jiao, et al. "MicroRNA-1285 regulates 17β-estradiol-inhibited immature boar Sertoli cell proliferation via adenosine monophosphate-activated protein kinase activation." Endocrinology (2015): en-2014.Read more>>
- Pan, Yi, et al. "RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src." Scientific Reports 6 (2016).Read more>>
- Gulikezi Maimaitirexiati. et al. Expression and correlation analysis of Skp2 and CBX7 in cervical cancer. J Clin Pathol. 2021 JulRead more>>
- Yun Zhao. et al. Exercise pretreatment alleviates neuroinflammation and oxidative stress by TFEB-mediated autophagic flux in mice with ischemic stroke. EXP NEUROL. 2023 Mar;:11438Read more>>
- Zhong Zhiwei. et al. Development of a prognostic model for anoikis and identifies hub genes in hepatocellular carcinoma. SCI REP-UK. 2023 Sep;13(1):1-19Read more>>
MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated(bs-1096R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Hela cells probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
293T lysates probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SKP2) polyclonal Antibody, Unconjugated (bs-1096R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Human 293T cell lysates probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.