bs-1096R-Cy3 [Conjugated Primary Antibody]
SKP2 Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SKP2

Immunogen Range: 351-424/424


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6502

Swiss Prot: Q13309

Source: KLH conjugated synthetic peptide derived from human Skp2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
  • Yang, Wei‐Rong, et al. "mTOR is involved in 17β‐estradiol‐induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." Molecular Reproduction and Development (2015).Read more>>
  • Yang, Wei‐Rong, et al. "mTOR is involved in 17β‐estradiol‐induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." Molecular Reproduction and Development (2015).Read more>>
  • Jiao, Zhang Jiao, et al. "MicroRNA-1285 regulates 17β-estradiol-inhibited immature boar Sertoli cell proliferation via adenosine monophosphate-activated protein kinase activation." Endocrinology (2015): en-2014.Read more>>
  • Pan, Yi, et al. "RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src." Scientific Reports 6 (2016).Read more>>
  • Gulikezi Maimaitirexiati. et al. Expression and correlation analysis of Skp2 and CBX7 in cervical cancer. J Clin Pathol. 2021 JulRead more>>
  • Yun Zhao. et al. Exercise pretreatment alleviates neuroinflammation and oxidative stress by TFEB-mediated autophagic flux in mice with ischemic stroke. EXP NEUROL. 2023 Mar;:11438Read more>>
  • Zhong Zhiwei. et al. Development of a prognostic model for anoikis and identifies hub genes in hepatocellular carcinoma. SCI REP-UK. 2023 Sep;13(1):1-19Read more>>
VALIDATION IMAGES