bs-1100R [Primary Antibody]
CD36 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD36

Immunogen Range: 51-150/472


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12491

Swiss Prot: Q08857

Source: KLH conjugated synthetic peptide derived from mouse CD36

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse heart lysates probed with Rabbit Anti-CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.


Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-CD36/PAS-4 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse spleen cells probed with \tCD36 Polyclonal Antibody, Unconjugated (bs-1100R) at [CNC.] for 30 minutes followed by incubation with a conjugated secondary (Secondary Catalog #) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).